Product Name

Aquaporin 2

Catalog #

SPC-503D

Alternative Names

AQP2, AQP-CD, AQPCD, WCH CD

Immunogen

Sequence N-CLKGLEPDTDWEEREVRRRQ (Rat)

Accession Number

NP_037041.2

Gene ID

25386

SwissProt

P34080

Applications

WB, IF

Host Species

Rabbit

Species Reactivity

Human, Mouse, Rat. Other species not yet tested.

Recommended Dilutions

1:1000-4000 (WB), 1:400 (IF)

Form

Affinity Purified

Storage Buffer

PBS pH 7.4, 50% glycerol and 0.09% sodium azide

Concentration

1mg/mL

Background Info

Detects ~28.8kDa. May detect larger glycosylated bands ~35-50kDa.

Conjugate

N/A

Package Size

100uL

Storage Temp

-20°C

Shipping Temp

Blue Ice, 4°C

Datasheet

SPC 503 Aquaporin 2 Transporters

Research Area

Transporters

Certificate of Analysis

0.5 μg/mL of SPC-503 was sufficient for detection of aquaporin 2 in 10μg of rat kidney tissue lysate by
colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Price

$285.00 USD Add to Cart Bulk Quote

Western blot analysis of Aquaporin 2 in rat kidney tissue using a 1 in 2000 dilution of SPC-503, showing glyco and non-glycosylated bands.


IF analysis of Aquaporin 2 in rat kidney tissues using a 1 in 200 dilution of SPC-503.  DAPI merge.

Research Background

Aquaporins selectively conduct water molecules in and out of the cell, while preventing the passage of ions and other solutes. Known as water channels, they are
integral membrane pore proteins (1, 2).
Aquaporin 2 is the vasopressin-regulated water channel of the apical membrane of collecting duct cells. It is located in kidney epithelial cells and usually lies dormant in intracellular vesicle membranes. When it is needed vasopressin binds to the cell surface vasopressin receptor, activating a signaling pathway that cause AQP2 containing vesicles to fuse with the plasma membrane so the AQP2 can be used by the cell (3). Defects in AQP2 area cause of an autosomal dominant form of nephrogenic diabetes insipidus (NDI) (4).

References

1. Gonen T., Walz T. (2006) Q. Rev. Biophys. 39(4): 361-396.
2. Knepper M.A. (1994) Proc Natl. Acad Sci. USA. 91(14): 6255-6258.
3. Lodish H.F. (2008) Molecular Cell Biology. New York: W.H. Freeman. Print. 445.
4. www.vivo.colostate.edu/hbooks/molecules/aquaporins.html

Cited References

1. Nils van der Lubbe, Christina H. Lim, Marcel E. Meima et al. Aldosterone does not require angiotensin II to activate NCC through a WNK4–SPAK–dependent pathway. Pflügers Archiv European Journal of Physiology. Published online: 3 May 2012. http://dx.doi.org/10.1007/s00424-012-1104-0