StressXpress®
Cortisol EIA Kit

Quantitative colorimetric detection of cortisol

Catalog No. SKT-201

5 out of 5 based on 1 customer rating
Species Reactivity ALL
Sample Types Dried Fecal Samples, EDTA Plasma, Heparin Plasma, Saliva, Serum, Tissue Culture Media, Urine
SKU: SKT-201 Categories: ,

Clear
SKT-201_Cortisol_EIA_Kit_Standard_Curve_Fig1.png
Graph of the Urine Spike Assay for the Cortisol EIA Kit StressXpress - SKT-201
Product Name Cortisol EIA Kit
Description

Quantitative colorimetric detection of cortisol

Species Reactivity Species Independent
Platform Microplate
Sample Types Dried Fecal Samples, EDTA Plasma, Heparin Plasma, Saliva, Serum, Tissue Culture Media, Urine
Detection Method Colorimetric Assay
Assay Type Competitive EIA (Enzyme Immune Assay)
Utility EIA kit used to quantitatively measure cortisol present in samples.
Sensitivity 30.3 pg/mL
Assay Range 100 - 3200 pg/ml
Precision Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1174.3 pg/mL, 6% CV Sample 2- 475.9 pg/mL, 5.6% CV Sample 3- 177.4 pg/mL, 14.7% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in ten assays run over multiple days by four operators. The mean and precision of the calculated Cortisol concentrations were: Sample 1- 1188.1 pg/mL, 7.2% CV Sample 2- 508.7 pg/mL, 6.3% CV Sample 3- 199.7 pg/mL, 10.9% CV
Incubation Time 90 Minutes
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet Lot. No. SC429429 , Kit Booklet Lot No. SC288128 , Kit Booklet Lot No SC187418 , Kit Booklet Lot No SC187760 , Kit Booklet Lot No SC587342 , MSDS , Steroid Solid Extraction Protocol , Saliva Sample Handling Instructions
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type EIA Kits
Assay Overview The Cortisol EIA kit is designed to quantitatively measure cortisol present in dried fecal extracts, saliva, urine, serum, plasma and tissue culture media samples. This kit measures total cortisol in extracted samples and in serum and plasma and free cortisol in saliva and urine. A cortisol standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture mouse antibodies. A cortisol-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a monoclonal antibody to cortisol to each well. After an 1 hour incubation the plate is washed and substrate is added. The substrate reacts with the bound cortisol-peroxidase conjugate. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the cortisol in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers.
Kit Overview
Component No.ItemQuantity / Size
SKC-201A Clear Microtitre 96 well Plate 1 Plate
SKC-201B Cortisol Standard 125 µl
SKC-201C StressXpress® Cortisol Antibody 3 ml
SKC-201D StressXpress® Cortisol Conjugate 50 µl
SKC-201E StressXpress® Cortisol Conjugate Diluent 5 ml
SKC-201F Assay Buffer 50 ml
SKC-201G Dissociation Reagent 1 ml
SKC-201H Wash Buffer Concentrate 50 ml
SKC-201I TMB Substrate 11 ml
SKC-201J Stop Solution 5 ml
SKC-201K Plate Sealer 1 each
Cite This Product Cortisol EIA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-201)

Biological Description

Alternative Names (11β)-11,17,21-trihydroxypregn-4-ene-3,20-dione EIA Kit
Research Areas Cancer, Cell Signaling, Neuroscience, Oxidative Stress
Scientific Background Cortisol, C21H30O5, (hydrocortisone, compound F) is the primary glucocorticoid produced and secreted by the adrenal cortex. It is often referred to as the “stress hormone” as it is involved in the response to stress and it affects blood pressure, blood sugar levels, and other actions of stress adaptation. Immunologically, cortisol functions as an important anti-inflammatory and plays a role in hypersensitivity, immunosuppression, and disease resistance (1). In the metabolic aspect, cortisol promotes gluconeogenesis, liver glycogen deposition, and the reduction of glucose utilization (2). Production of cortisol follows an ACTH-dependent circadian rhythm, with a peak level in the morning and decreasing levels throughout the day. Most serum cortisol, all but about 4%, is bound to proteins including corticosteroid binding globulin and serum albumin (1,3). Only free cortisol is available to most receptors and it is through these receptors that physiological processes are modulated. Abnormal cortisol levels are being evaluated for correlation with a variety of different conditions, such as prostate cancer (4), depression (5), and schizophrenia (6). It is already known that abnormal levels of cortisol are involved in Cushing’s Syndrome and Addision’s disease (7).
References 1. E. Friess, et al., Eur J Clin Invest, 2000, 30, Suppl 3:46-50.
2. Freeman, Scott, 2002. Biological Science. Prentice Hall; 2nd Pkg edition (December 30, 2004).
3. C. Longscope., J. Endocrinology, 1996, , Suppl S125-S127.
4. J. Herbert, Lancet, 1995 345, 1193-1194.
5. A. Michael, et al., Biol. Psychiatry, 2000, 48, 989-95.
6. C.R. Dequet and D.J. Wallace, Current Opin. Ivest. Drugs, 2001, 8, 1045-53.
7. W.M. Jeffries, Med. Hypotheses, 1998, 51, 114-4.

Product Images

<p>Typical Standard Curve for the Cortisol EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-201. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 100 – 3200 pg/ml.</p>

Typical Standard Curve for the Cortisol EIA Kit (Enzyme Immunoassay) StressXpress® – SKT-201. Assay Type: Sandwich EIA. Detection Method: Colorimetric Assay. Assay Range: 100 – 3200 pg/ml.

<p>Linearity was determined by taking two human urine samples diluted 1:140, one with a low diluted cortisol level of 163.9 pg/mL and one with a higher diluted level of 2,974.9 pg/mL and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.</p>

Linearity was determined by taking two human urine samples diluted 1:140, one with a low diluted cortisol level of 163.9 pg/mL and one with a higher diluted level of 2,974.9 pg/mL and mixing them in the ratios given below. The measured concentrations were compared to the expected values based on the ratios used.

Reviews

1 review for Cortisol EIA Kit

  1. 5 out of 5

    :

    Based on validation through cited publications.

Add a review

Your email address will not be published. Required fields are marked *