ELISA Kits - Cellular Stress, Heat Shock Proteins (HSP), Oxidative Stress

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Product Name: Hsp90 Antibody and Protein Pack
Catalog #: SPK-210
Alternative Names: Hsp84, Hsp86, Hsp90A, Hsp90AA1, Hsp90AB1, Hsp90B, HspC1, HspC2, HspCAL1, HspCAL4
Size: 13x 25ug
Type: Kit
Description: 13 Antibodies and Proteins to Hsp90, see Antibody and Protein Details Tab
Species Reactivity: Multiple
Sample Type: N/A
Research Area: Chaperones, Heat Shock
Incubation Time: N/A
Sensitivity: N/A
Assay Range: N/A
Storage Temp: -20°C
Shipping Temp: Blue Ice, 4°C
Datasheet: SPK 210
Price: $645.00 USD Add to Cart Bulk Quote

Product (13 x 25ug)	Cited Applications	Species Cross-reactivity	Format/ Concentration

Hsp90alpha Monoclonal Antibody, K41009 Cat# SMC-108	WB, IP, ELISA, IHC	Human (alpha-specific), Rat	Mouse IgG2a in PBS pH 7.2, 50% glycerol, 0.09% sodium azide. Protein G Purified. 1mg/mL
Hsp90 Monoclonal Antibody, K41220A Cat# SMC-135	WB, IHC, ELISA	Human (beta-specific), Rat, Liver, S.cerevisiae, S.pombe. Limited or no cross-reactivity to rice or P.caudatum.	Mouse IgG2a in PBS pH 7.2, 50% glycerol, 0.09% sodium azide. Protein G Purified. 1mg/mL
Hsp90beta Monoclonal Antibody, K3701 Cat# SMC-136	WB,ELISAP, IHC	Human (beta-specific- does not cross react with alpha), Rat.	Mouse IgM in PBS pH 7.2, 50% glycerol, 0.09% sodium azide. Protein G Purified. 1mg/mL
Hsp90 Monoclonal Antibody, D7alpha Cat# SMC-137	IHC, IP, WB	Bovine, Chicken, Human, Mouse, Porcine, Rat, Rabbit.	Mouse IgG1 in PBS, 50% glycerol, 0.09% sodium azide. Protein G Purified. 1mg/mL
Hsp90 Monoclonal Antibody, AC16 Cat# SMC-112	IHC, WB	Human, Rabbit, Rat, Mouse, Chicken, Achyla, Wheat germ, Sf9 cell line.	Mouse IgG2b in PBS pH 7.4, 50% glycerol, 0.09% azide. Protein G Purified. 1mg/mL
Hsp90 complex Monoclonal Antibody, 8D3 Cat# SMC-109	IP	Human, Mouse, Rat, Rabbit. Other species not yet tested.	Mouse IgM in 50% glycerol, 0.09% azide. Protein G Purified. 1mg/mL
Hsp90beta Polyclonal Antibody Cat# SPC-177	WB, IP, ELISA, IHC	Human, Rat, Mouse	Whole Rabbit Serum
Hsp90 Polyclonal Antibody Cat# SPC-104	WB, ELISA, IP, IHC	Human, Mouse, Rat.	Whole Rabbit Serum
Hsp90alpha Monoclonal Antibody, 2G5.G3 Cat# SMC-147	WB, ELISA, IP, IHC, ICC,IF	Human, Mouse, Rat. Other species not yet tested.	Mouse IgG in PBS pH 7.2, 50%glycerol, 0.09% azide. Protein G Purified. 1mg/mL
Hsp90 Monoclonal Antibody, 4FE.E8 Cat# SMC-149	WB, IP, IF, IHC, ELISA, ICC	Human, Hamster, Rat. Other species not yet tested.	Mouse IgG1 in PBS pH7.2, 50% glycerol, 0.09% sodium azide. Protein G Purified. 1mg/mL
Hsp90 Monoclonal Antibody, H9010 Cat# SMC-107	WB, IP, ELISA, IHC	Human (beta specific), Rabbit (beta specific), Chicken (alpha/beta), Rat, Canine	Mouse IgG2a in PBS pH7.2, 50% glycerol, 0.09% azide. Protein G Purified. 1mg/mL
Recombinant Hsp90 alpha Protein Cat# SPR-101	WB Control	N/A	Human Hsp90alpha in 50mM Tris/HCl pH7.5, 5mM Bme, 0.3M NaCl, 10% glycerol. Mulit-step chromatographically purified. 0.5mg/mL
Recombinant Hsp90 beta Protein (endotoxin free) Cat# SPR-102	WB Control, Chaperone Assays, Inhibitors/ binding assays, ELISA reference standard	N/A	Human Hsp90beta in 20mM Tris pH7.5, 175mM NaClm 0.1mM EDTA, 10% glycerol, 1mM DTT. Multi-step chromatographically purified. 1.7mg/mL

Research Background: Hsp90 is a highly conserved and essential stress protein that is expressed in all eukaryotic cells. From a functional perspective, hsp90 participates in the folding, assembly, maturation, and stabilization of specific proteins as an integral component of a chaperone complex (1-4). Despite its label of being a heat-shock protein, hsp90 is one of the most highly expressed proteins in unstressed cells (1–2% of cytosolic protein). It carries out a number of housekeeping functions – including controlling the activity, turnover, and trafficking of a variety of proteins. Most of the hsp90- regulated proteins that have been discovered to date are involved in cell signaling (5-6). The number of proteins now know to interact with Hsp90 is about 100. Target proteins include the kinases v-Src, Wee1, and c-Raf, transcriptional regulators such as p53 and steroid receptors, and the polymerases of the hepatitis B virus and telomerase.5 When bound to ATP, Hsp90 interacts with co-chaperones Cdc37, p23, and an assortment of immunophilin-like proteins, forming a complex that stabilizes and protects target proteins from proteasomal degradation. In most cases, hsp90-interacting proteins have been shown to co-precipitate with hsp90 when carrying out immunoadsorption studies, and to exist in cytosolic heterocomplexes with it. In a number of cases, variations in hsp90 expression or hsp90 mutation has been shown to degrade signaling function via the protein or to impair a specific function of the protein (such as steroid binding, kinase activity) in vivo. Ansamycin antibiotics, such as geldanamycin and radicicol, inhibit hsp90 function (7).
References: 1. Nemoto T., et al. (1997) J.Biol Chem. 272: 26179-26187.
2. Minami Y., et al. (1991), J.Biol Chem. 266: 10099-10103.
3. Arlander S.J.H., et al. (2003) J Biol Chem 278: 52572- 52577.
4. Pearl H., et al. (2001) Adv Protein Chem 59:157-186.
5. Neckers L., et al. (2002) Trends Mol Med 8:S55-S61.
6. Pratt W., Toft D. (2003) Exp Biol Med 228:111-133.
7. Pratt W., Toft D. (1997) Endocr Rev 18:306–360.
8. Pratt W.B. (1998) Proc Soc Exptl Biol Med 217: 420–434.
9. Whitesell L., et al. (1994) Proc Natl Acad Sci USA 91: 8324– 8328.
10. Barent R. L. (1998) Mol. Endocrinol. 12: 342-354
11. Lo. M.A. (1998) EMBO J. 17: 6879-6887.

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