Proteins - Purified Proteins, Heat Shock Proteins, Oxidative Cellular Stress

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Product Name: Grp78 (Bip, Recombinant, Human, His-tag)
Catalog #: SPR-107C
Alternative Names: BIP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein
Type: Recombinant Protein
Accession Number: NM_005347
Gene ID: 3309
SwissProt: P11021
Applications: ATPase Assay, WB control, Binding Assay, ELISA reference standard
Species: Human
Conjugate/Tag: His-tag
Form: Affinity Purifiied using His tag
Storage Buffer: 20mM Tris, 50mM KCl, 5mM MgCl2, 1mM DTT, pH7.5
Concentration: 1.87mg/mL
Certificate of Analysis: This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 2.3μM phosphate liberated/hr/μg protein in a 200μl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Background Info: Molecular weight of approximately 78kD
Package Size: 2x 100ug
Other Information: ATPase active
Storage Temp: -20°C
Shipping Temp: Dry ice, Blue Ice or 4°C
Datasheet: SPR 107 Glucose Regulated Protein 78 BIP (GRP78)
Research Area: Chaperones, Heat Shock, Trafficking
Price: $469.00 USD Add to Cart

SDS PAGE of Grp78 protein.

Research Background: GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
References: 1. Cho, S. et al (2007). Mol Cell Biol 27(1): 368-83.
2. Yang, Y. et al. (1998) J Biol Chem 273:25552-25555.
3. Luo, S. et al (2006) 26 (15): 5688-97.
4. Yu, Z. et al. (1999) Exp Neurol. 15: 302-314.
5. Koomagi, R. et al. (1999) Anticancer Res. 19: 4333-4336.
6. Laquerre, S. et al. (1998) J. Virology 72: 4940-4949.
7. Dong, D. et al. (2005) Cancer Res 65(13): 5785-91.

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