Product Name

CDC37 (Recombinant, Human, His-tag)

Catalog #

SPR-304C

Type

Recombinant Protein

Accession Number

NP_008996.1

Gene ID

11140

SwissProt

Q16543

Applications

WB control, functional/activity studies

Species

Human

Conjugate/Tag

His-tag

Form

Molecular weight of approximately 44.5kDa.

Storage Buffer

25mM Tris buffer pH7.2, 150mM NaCl, 0.1 DTT in 50% glycerol

Concentration

1mg/mL

Certificate of Analysis

This product has been certified >90% pure using SDS-PAGE analysis.

Package Size

200ug

Storage Temp

-20°C

Shipping Temp

Blue Ice, 4°C

Datasheet

SPR 304 CDC37 (Human) Heat Shock

Research Area

Heat Shock

Price

$495.00 USD Add to Cart Bulk Quote

Western blot analysis of CDC37 (Human), showing specificity at ~44.5kDa.


Western blot analysis showing pull down data for CDC37 (Human) with Hsp90, as indicated by the arrow.


Hela cells were lysed (50mM TrispH7.2, 100mM NaCl, 1mM MgCl2, 1%Tx-100, PIC) and centrifuged to generate a PNS. 25ug of recombinant His-tagged cdc37 was added to PNS and incubated for 1hr at 4°C and then NiNTA beads (25ul) were added for 1hr. Adsorbed protein complexes were analyzed by SDS-PAGE followed by Coomassie  staining. First lane is background adsorption to beads with no recombinant protein added.

Research Background

Hsp90 co-chaperone Cdc37 is a protein that is encoded by the CDC37 gene. It has been found to form complexes with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF1, MOK and elF-2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases (1, 2). CDC37 is necessary for maintaining prostate tumor cell growth and represents a novel target in the exploration for multi-targeted therapies (3, 4).

References

1. Dai K, Kobayashi R., Beach D. (1996) J Biol Chem. 271(36): 22030-22034.
2. Stepanova L, Leng X., Parker S.B., Harper J.W. (1996) Genes Dev. 10(12): 1491-1502.
3. Kimura Y., et al. (1997) Genes Dev. 11(14): 1775-1185.
4. Gray P.J., et al. (2008) Nat Rev Cancer. 8(7): 491-495.