Anti-HSP70 Antibody [C92F3A-5]

Mouse Anti-Human HSP70 Monoclonal IgG

Catalog No. SMC-100

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Species Reactivity , Hu , Ms , Rt , Bv , C.el , Dg , Ck , Dr , Cp , GP , Hm , Mk , Pg , Rb , Sh
Applications WB IHC ICC/IF FCM IP
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USD $40.00USD $417.00

SKU: SMC-100 Categories: , .

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SMC-100_Hsp70_Antibody_C92_IHC_Mouse_colon-carcinoma_40x_1.png
Mouse Anti-Hsp70 Antibody [C92] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Mouse Heat Shocked Melanoma cells (SMC-100)Mouse Anti-Hsp70 Antibody [C92] used in Immunohistochemistry (IHC) on Human colon carcinoma (SMC-100)Mouse Anti-Hsp70 Antibody [C92] used in Western Blot (WB) on Human cell lysates from various cell lines (SMC-100)Mouse Anti-Hsp70 Antibody: FITC [C92] used in Fluorescence Activated Cell Sorting (FACS) on Mouse Heat Shocked CD3+ CD8+ T cells  (SMC-100)

Overview

Product Name HSP70 Antibody
Description

Mouse Anti-Human HSP70 Monoclonal IgG

Species Reactivity Dog, Human, Monkey, Mouse, Rat, Bovine, Carp (Cypriniformes), Chicken, Fruit Fly (Drosophila melanogaster), Guinea Pig (Cavia porcellus), Hamster, Nematode (Caenorhabditis elegans), Pig, Rabbit, Sheep
Applications , WB , IHC , ICC/IF , ELISA , FCM , FACS , IEM , Bl , AM
Antibody Dilution WB (1:1000), IHC (1:10000), ICC/IF (1:1000), FACS (1:1000); optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Human HSP70
Concentration 1 mg/ml
Conjugates Alkaline Phosphatase, APC, ATTO 390, ATTO 488, ATTO 565, ATTO 594, ATTO 633, ATTO 655, ATTO 680, ATTO 700, Biotin, FITC, HRP, PE/ATTO 594, PerCP, RPE, Streptavidin, Unconjugated
APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

 APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

  ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

 ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

 ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

 ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

 ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

 PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

 R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.1% sodium azide
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number C92F3A-5
Isotype IgG
Specificity Detects ~70kDa. Does not cross-react with HSC70 (HSP73).
Cite This Product Mouse Anti-Human HSP70 Monoclonal, Clone C92F3A-5 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SMC-100)
Certificate of Analysis 1 µg/ml of SMC-100 was sufficient for detection of HSP70 in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Biological Description

Alternative Names HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody
Research Areas Cancer, Heat Shock
Cellular Localization Cytoplasm
Accession Number NP_005336.3
Gene ID 3303
Swiss Prot P08107
Scientific Background HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5).
All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
References 1. Welch W.J. and Suhan J.P. (1986) J Cell Biol. 103: 2035-2050.
2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J.Mol. Evol. 38(1): 1-17.
3. Rothman J. (1989) Cell 59: 591-601.
4. DeLuca-Flaherty et al. (1990) Cell 62: 875-887.
5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294.
6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
7. Galan A., et al. (2000) J. Biol. Chem. 275: 11418-11424.
8. Kondo T., et al. (2000) J. Biol. Chem. 275: 8872-8879.
9. Misaki T., et al. (1994) Clin. Exp. Immun. 98: 234-239.
10. Pockley A.G., et al. (1998) Immunol. Invest. 27: 367-377.
11. Moon I.S., et al. (2001) Cereb Cortex 11(3): 238-248.
12. Dressel et al. (2000) J. Immunol. 164: 2362-2371.
13. Verma A.K., et al. (2007) Fish and Shellfish Immunology. 22(5): 547-555.
14. Banduseela V.C., et al. (2009) Physiol Genomics. 39(3): 141-159.

Product Images

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Ceter and Inst. Of Oncology, Poland.

Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.

Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92 (SMC-100). Load: 15 µg protein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (SMC-100) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92 (SMC-100). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (SMC-100) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.

Product Citations (55)

Western Blot

The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis.

Qiao, A., Jin, X., Pang, J., Moskophidis, D. and Mivechi, N.f. (2017) J Cell Biol. [Epub ahead of print].

PubMed ID: 28183717 Reactivity: Mouse Applications: Western Blot

TRIM32-Cytoplasmic-Body Formation Is an ATP-Consuming Process Stimulated by HSP70 in Cells.

Kawaguchi, Y. et al. (2017) PLoS One. 12(1):e0169436.

PubMed ID: 28052117 Reactivity: Human Applications: Western Blot

Heat shock protein 90 ensures efficient mumps virus replication by assisting with viral polymerase complex formation

Katoh, H. et al. (2017) J Virol. pii: JVI.02220-16.

PubMed ID: 28053100 Reactivity: Human Applications: Western Blot

Senkyunolide I attenuates oxygen-glucose deprivation/reoxygenation-induced inflammation in microglial cells.

Hu, Y.Y. et al. (2016) Brain Res. 1649(Pt A):123-131.

PubMed ID: 27524398 Reactivity: Mouse Applications: Western Blot

Effects of Long-Term Exposure to 60 GHz Millimeter-Wavelength Radiation on the Genotoxicity and Heat Shock Protein (Hsp) Expression of Cells Derived from Human Eye.

Koyama, S. et al. (2016) Int J Environ Res Public Health. 13(8). pii: E802.

PubMed ID: 27509516 Reactivity: Human Applications: Western Blot

Twenty Four-Hour Exposure to a 0.12 THz Electromagnetic Field Does Not Affect the Genotoxicity, Morphological Changes, or Expression of Heat Shock Protein in HCE-T Cells.

Koyama, S. et al. (2016) Int J Environ Res Public Health. 13(8). pii: E802.

PubMed ID: 27527204 Reactivity: Human Applications: Western Blot

Heat Shock Factor 1 is a Substrate for p38 Mitogen-Activated Protein Kinases.

Dayalan Naidu, S. et al. (2016) Mol Cell Biol. 36(18):2403-17.

PubMed ID: 27354066 Reactivity: Human Applications: Western Blot

Expression of Heat Shock Proteins in Human Fibroblast Cells under Magnetic Resonant Coupling Wireless Power Transfer.

Mizuno, K., Shinohara, N. and Miyakoshi, J. (2015) Energise. 8(10): 12020-12028.

PubMed ID: N/A Reactivity: Human Applications: Western Blot

Critical Illness Myopathy: Understanding different effects on muscle fibre function.

Ogilvie, H. (2015) Karolinska Institutet. PhD Dissertation.

PubMed ID: N/A Reactivity: Rat Applications: Western Blot

Heat shock protein 70 regulates degradation of the mumps virus phosphoprotein via the ubiquitin-proteasome pathway.

Katoh, H. et al. (2014) J Virol. 89(6):3188-99.

PubMed ID: 25552722 Reactivity: Human Applications: Western Blot

Heat-induced expression of the immediate-early gene IER5 and its involvement in the proliferation of heat-shocked cells.

Ishikawa, Y. and Sakurai, H. (2014) FEBS J. 282(2):332-40.

PubMed ID: 25355627 Reactivity: Human Applications: Western Blot

Prostaglandin E Synthase Interacts with Inducible Heat Shock Protein 70 After Heat Stress in Bovine Primary Dermal Fibroblast Cells.

Richter, C., Viergutz, T., Schwerin, M. and Weitzel, J.M. (2014) Cytometry A. 87(1):61-7.

PubMed ID: 25412999 Reactivity: Bovine Applications: Western Blot

Inhibition of autophagy, lysosome and VCP function impairs stress granule assembly.

Seguin, S.J. et al. (2014) Cell Death Differ. 21(12):1838-51.

PubMed ID: 25034784 Reactivity: Human Applications: Western Blot

Fasting Enhances TRAIL-Mediated Liver Natural Killer Cell Activity via HSP70 Upregulation.

Dang, V.T.A. et al. (2014) PLoS One. 9(10): e110748.

PubMed ID: 25356750 Reactivity: Mouse Applications: Western Blot

Direct binding of the Alu binding protein dimer SRP9/14 to 40S ribosomal subunits promotes stress granule formation and is regulated by Alu RNA

Berger, A. et al. (2014) Nucleic Acids Res. 42(17):11203-17.

PubMed ID: 25200073 Reactivity: Human Applications: Western Blot

Therapeutic Inducers of the HSP70/HSP110 Protect Mice Against Traumatic Brain Injury.

Eroglu, B. et al. (2014) J Neurochem. 130(5):626-41.

PubMed ID: 24903326 Reactivity: Mouse Applications: Western Blot

Moderate Alcohol Induces Stress Proteins HSF1 and hsp70 and Inhibits Proinflammatory Cytokines Resulting in Endotoxin Tolerance.

Muralidharan, S. et al. (2014) J Immunol. 193(4):1975-87.

PubMed ID: 25024384 Reactivity: Human Applications: Western Blot

Masseter muscle myofibrillar protein synthesis and degradation in an experimental critical illness myopathy model.

Akkad, H., Corpeno, R., Larsson L. (2014) PLoS One. 9(4): e92622.

PubMed ID: 24705179 Reactivity: Rat Applications: Western Blot

Structure-Activity Relationships for Withanolides as Inducers of the Cellular Heat-Shock Response.

Wijeratne, E.M., et al. (2014) J Med Chem. 57(7):2851-63.

PubMed ID: 24625088 Reactivity: Mouse Applications: Western Blot

Detection of constitutive and inducible HSP70 proteins in formalin fixed human brain tissue.

Preusse-Prange, A., Modrow, J.H., Schwark, T., von Wurmb-Schwark, N. (2014) Forensic Sci Int. 235:62-7.

PubMed ID: 24447452 Reactivity: Human Applications: Western Blot

Overexpression of Heat Shock Protein 72 Attenuates NF-κB Activation Using a Combination of Regulatory Mechanisms in Microglia.

Sheppard, P.W., Sun, X., Khammash, M., Giffard, R.G. (2014) PLoS Comput Biol. 10(2):e1003471.

PubMed ID: 24516376 Reactivity: Mouse Applications: Western Blot

Effects of corticosteroids in the development of limb muscle weakness in a porcine intensive care unit model.

Aare, S. et al. (2013) Physiol Genomics. 45 (8): 312-320.

PubMed ID: 23429211 Reactivity: Pig Applications: Western Blot

Iron modulates cell survival in a Ras- and MAPK-dependent manner in ovarian cells.

Bauckman, K.A., Haller, E., Flores. I., and Nanjundan, M. (2013) Cell Death Dis. 4.e592.

PubMed ID: 23598404 Reactivity: Human Applications: Western Blot

Molecular and Cellular Networks in Critical Illness Associated Muscle Weakness: Skeletal Muscle Proteostasis in the Intensive Care Unit.

Banduseela, V.C. (2012) Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Neuroscience. PhD Dissertation

PubMed ID: N/A Reactivity: Pig Applications: Western Blot

Influences of temperature, oxidative stress, and phosphorylation on binding of heat shock proteins in skeletal muscle fibers.

Larkins, N.T., Murphy, R.M., and Lamb, G.D. (2012) Am J Physiol Cell Physiol. 303 (6): C654-C665.

PubMed ID: 22763123 Reactivity: Rat Applications: Western Blot

DnaJA1 Antagonizes Constitutive Hsp70-Mediated Stabilization of Tau.

Abisambra, J.F., et al. (2012) J.Mol.Biol. 421: 653-661.

PubMed ID: 22343013 Reactivity: Human Applications: Western Blot

Hsp70 Promotes Epithelial Sodium Channel Functional Expression by Increasing Its Association with Coat Complex II and Its Exit from Endoplasmic Reticulum.

Chanoux, R.A. et al. (2012) J Biol Chem. 287, 19255-19265.

PubMed ID: 22496374 Reactivity: Dog Applications: Western Blot

Using the Heat-Shock Response To Discover Anticancer Compounds that Target Protein Homeostasis.

Santagata, S. et al. (2012) ACS Chem.Biol. 7 (2): 340-349.

PubMed ID: 22050377 Reactivity: Human Applications: Western Blot

Absolute amounts and diffusibility of HSP72, HSP25, and αB-crystallin in fast- and slow-twitch skeletal muscle fibers of rat.

Larkins, N.T., Murphy, R.M., and Lamb, G.D. (2011) Am J Physiol Cell Physiol. 302 (1): C228-C239.

PubMed ID: 21975426 Reactivity: Rat Applications: Western Blot

Mechanisms underlying the sparing of masticatory versus limb muscle function in an experimental critical illness model.

Aare, S. et al. (2011) Physiol Genomics. 43 (24): 1334-1350.

PubMed ID: 22010006 Reactivity: Pig Applications: Western Blot

Heat Shock Protein 70 Prevents both Tau Aggregation and the Inhibitory Effects of Preexisting Tau Aggregates on Fast Axonal Transport.

Patterson, K.R. et al. (2011) Biochem. 50 (47): 10300-10310.

PubMed ID: 22039833 Reactivity: Human Applications: Western Blot

Withaferin A Analogs and Uses Thereof.

Gunatilaka, L., Lindquist, S.L., Whitesell, L.,Wijeratne, E.M.K., and Xu, Y. (2011) United States Patent Application US20110230551 A1.

PubMed ID: N/A Reactivity: Mouse Applications: Western Blot

Preferential skeletal muscle myosin loss in response to mechanical silencing in a novel rat intensive care unit model: underlying mechanisms.

Ochala, J. et al. (2011) J Physio. 589 (8): 2007-2026.

PubMed ID: 21320889 Reactivity: Rat Applications: Western Blot

Co-overexpression of Bag-1 and heat shock protein 70 in human epidermal squamous cell carcinoma: Bag-1-mediated resistance to 5-fluorouracil induced apoptosis.

Wood, J. et al. (2011) Br J Cancer. 104 (9): 1459-1471.

PubMed ID: 21522149 Reactivity: Human Applications: Western Blot

Effects of HSP70 on the compression force-induced TNF-α and RANKL expression in human periodontal ligament cells.

Mitsuhashi, M., Yamaguchi, M., Kojima, T., Nakajima, R., Kasai, K. (2011) Inflammation Research. 60 (2): 187-194.

PubMed ID: 20924639 Reactivity: Human Applications: Western Blot

Deciphering Human Heat Shock Transcription Factor 1 Regulation via Post-Translational Modification in Yeast.

Batista-Nascimento, L., Neef, D.W., Liu, P.C.C., Rodrigues-Pousada, C., Thiele, D.J. (2011) PLoS One. 6 (1): e15976.

PubMed ID: 21253609 Reactivity: Mouse Applications: Western Blot

Deciphering Human Heat Shock Transcription Factor 1 Regulation via Post-Translational Modification in Yeast.

Batista-Nascimento, L., Neef, D.W., Liu, P.C.C., Rodrigues-Pousada, C., Thiele, D.J. (2011) PLoS One. 6 (1): e15976.

PubMed ID: 21253609 Reactivity: Human Applications: Western Blot

Tamm-Horsfall protein and urinary exosome isolation.

Fernández-Llama, P. et al. (2010) Kidney Int. 77, 736-742.

PubMed ID: 20130532 Reactivity: Human Applications: Western Blot

Gene expression and muscle fiber function in a porcine ICU model.

Banduseela, V.C. et al. (2009) Physiol Genomics. 39 (3): 141-159.

PubMed ID: 19706692 Reactivity: Pig Applications: Western Blot

2,3-Dihydrowithaferin A-3β-O-sulfate, a new potential prodrug of withaferin A from aeroponically grown Withania somnifera.

Xu, Y. et al. (2009) Bioorg Med Chem. 17 (6): 2210-2214.

PubMed ID: 19056281 Reactivity: Mouse Applications: Western Blot

Identification of Phosphorylation-Dependent Binding Partners of Aquaporin-2 Using Protein Mass Spectrometry.

Zwang, N.A. et al. (2009) J.Proteome Res. 8 (3): 1540-1554.

PubMed ID: 19209902 Reactivity: Rat Applications: Western Blot

Immunoprecipitation

Prostaglandin E Synthase Interacts with Inducible Heat Shock Protein 70 After Heat Stress in Bovine Primary Dermal Fibroblast Cells.

Richter, C., Viergutz, T., Schwerin, M. and Weitzel, J.M. (2014) Cytometry A. 87(1):61-7.

PubMed ID: 25412999 Reactivity: Bovine Applications: Immunoprecipitation

Macrophage inflammatory protein derivative ECI301 enhances the alarmin-associated abscopal benefits of tumor radiotherapy.

Kanegassaki, S., Matsushima, K., Shiraishi, K., Nakagawa, K. and Tsuchiya, T. (2014) Cancer Res. 74(18):5070-8.

PubMed ID: 25038226 Reactivity: Mouse Applications: Immunoprecipitation

Immunohistochemistry

Can hypoxia enhance sexual arousal?-Molecular-biological analysis of the hypothalamus in male rats placed with oestrous female rats under hypoxic conditions.

Inoue, H., Yoshida, M. Nishio, H. and Tatsumi, S. (2016) Int J Clin Exp Med. 9(10):19512-19520

PubMed ID: N/A Reactivity: Rat Applications: Immunohistochemistry

Detection of constitutive and inducible HSP70 proteins in formalin fixed human brain tissue.

Preusse-Prange, A., Modrow, J.H., Schwark, T., von Wurmb-Schwark, N. (2014) Forensic Sci Int. 235:62-7.

PubMed ID: 24447452 Reactivity: Human Applications: Immunohistochemistry

Co-overexpression of Bag-1 and heat shock protein 70 in human epidermal squamous cell carcinoma: Bag-1-mediated resistance to 5-fluorouracil induced apoptosis.

Wood, J. et al. (2011) Br J Cancer. 104 (9): 1459-1471.

PubMed ID: 21522149 Reactivity: Human Applications: Immunohistochemistry

Flow Cytometry

Prostaglandin E Synthase Interacts with Inducible Heat Shock Protein 70 After Heat Stress in Bovine Primary Dermal Fibroblast Cells.

Richter, C., Viergutz, T., Schwerin, M. and Weitzel, J.M. (2014) Cytometry A. 87(1):61-7.

PubMed ID: 25412999 Reactivity: Bovine Applications: Flow Cytometry

Immunocytochemistry/Immunofluorescence

Heat shock protein 70 regulates degradation of the mumps virus phosphoprotein via the ubiquitin-proteasome pathway.

Katoh, H. et al. (2014) J Virol. 89(6):3188-99.

PubMed ID: 25552722 Reactivity: Human Applications: Immunocytochemistry/Immunofluorescence

Other Citations

Fasting Enhances TRAIL-Mediated Liver Natural Killer Cell Activity via HSP70 Upregulation.

Dang, V.T.A. et al. (2014) PLoS One. 9(10): e110748.

PubMed ID: 25356750 Reactivity: Mouse Applications: Protein Inhibition

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity: Human Applications: Antibody Microarray

Biomarker Analysis with Grating Coupled Surface Plasmon Coupled Fluorescence.

Mendoza, A., Dias, J.A., Zeltner, T. and Lawrence, D.A. (2014) J Adv Bio & Biotech. 1(1): 1-22.

PubMed ID: N/A Reactivity: Mouse Applications: Antibody Microarray

Heat Shock Protein 70 Prevents both Tau Aggregation and the Inhibitory Effects of Preexisting Tau Aggregates on Fast Axonal Transport.

Patterson, K.R. et al. (2011) Biochem. 50 (47): 10300-10310.

PubMed ID: 22039833 Reactivity: Human Applications: Electron Microscopy

Associations of HSP90 Client Proteins in Human Breast Cancer.

Shipp, C., Watson, K., and Jones, G.L. (2011) Anticancer Research. 31 (6): 2095-2101.

PubMed ID: 21737627 Reactivity: Human Applications: Immunoprecipitation

Reviews

1 review for HSP70 Antibody

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    Based on validation through cited publications.

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