StressXpress®
HSP60 ELISA Kit

Colorimetric detection of HSP60

Catalog No. SKT-110

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Species Reactivity , Hu
Sample Types Cell lysates, Serum, Tissue

USD $514.00

SKU: SKT-110 Categories: , .

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SKT-110_HSP60_ELISA_Kit_Typical_Standard_Curve_Lot-TH585578

Overview

Product Name HSP60 ELISA Kit
Description

Colorimetric detection of HSP60

Species Reactivity Human
Platform Microplate
Sample Types Cell lysates, Serum, Tissue
Detection Method Colorimetric Assay
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Utility ELISA kit used to quantitate HSP60 concentration in samples.
Sensitivity 1.73 ng/ml
Assay Range 11 - 700 ng/ml
Incubation Time 30 minutes
Number of Samples 40 samples in duplicate
Other Resources , Kit Booklet Lot No. BH585599 , Kit Booklet Lot No. TH585578 , Kit Booklet Lot No. 160427 , MSDS

Properties

Storage Temperature 4ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Assay Overview 1. Prepare Standard and samples in Standard and Sample Diluent.
2. Add 100 µL of Standard or sample to appropriate wells.
3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour.
4. Wash plate four times with 1X Wash Buffer.
5. Add 100 µL of Biotinylated Antibody Working Solution to each well.
6. Cover plate with Plate Sealer and incubate at 37°C for 1 hour.
7. Wash plate four times with 1X Wash Buffer.
8. Add 100 µL of Streptavidin-HRP Working Solution to each well.
9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes.
10. Wash plate four times with 1X Wash Buffer.
11. Add 100 µL of TMB Substrate to each well.
12. Develop the plate in the dark at room temperature for 30 minutes.
13. Stop reaction by adding 100 µL of Stop Solution to each well.
14. Measure absorbance on a plate reader at 450 nm.
Kit Components
Component No. Item Quantity / Size
SKC-110A Anti-Hsp60 Immunoassay Plate 1 Plate
SKC-110B 5X Hsp60 Extraction Reagent 1 vial/10 ml
SKC-110C Recombinant Hsp60 Standard 1 vial
SKC-110D Standard and Sample Diluent 1 vial/ 50 ml
SKC-110E 10X Wash Buffer Concentrate 1 vial/100 ml
SKC-110F Anti-Hsp60 Biotinylated Antibody Concentrate 1 vial/150 µl
SKC-110G Anti-Hsp60 Biotinylated Antibody Diluent 1 vial/ 13 ml
SKC-110H Streptavidin: HRP Concentrate 1 vial/150 µl
SKC-110I Streptavidin: HRP Diluent 1 vial/ 13 ml
SKC-110J TMB Substrate 1 vial/ 13 ml
SKC-110K Stop Solution 1 vial/ 13 ml
Cite This Product HSP60 ELISA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-110)

Biological Description

Alternative Names CPN60 ELISA Kit, GROEL ELISA Kit, HLD4 ELISA Kit, HSP 60 ELISA Kit, HSP65 ELISA Kit, HSPD1 ELISA Kit, HuCHA60 ELISA Kit, SPG 13 ELISA Kit
Research Areas Cancer, Heat Shock
Scientific Background In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP,iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimers, coronary artery diseases, MS, and diabetes (6-9).
References 1. Hartl F.U. (1996) Nature. 381: 571-579.
2. Bukau B. and Horwich, A.L. (1998) Cell. 92: 351-366.
3. Hartl F.U. and Hayer-Hartl, M. (2002) Science. 295: 1852-1858.
4. Jindal S., et al. (1989) Molecular and Cellular Biology. 9: 2279-2283.
5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71.
6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938.
7. Gupta S. and Knowlton A.A. J. Cell Mol. Med. 9: 51-58.
8. Deocaris C.C., et al. (2006) Cell Stress Chaperones. 11: 116-128.
9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.

Product Images

Typical Standard Curve for the HSP60 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-110. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 7 – 450 ng/mL.

Typical Standard Curve for the HSP60 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-110. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 11 – 700 ng/ml.

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