Tau

StressMarq Biosciences has developed an extensive range of fibrillar, oligomeric and monomeric protein preparations for use in neurodegenerative disease research including alpha synucleinbeta synucleingamma synucleintauamyloid betaSOD1 and TTR. Our goal is to be the world leader in the development and supply of active, pathology-inducing protein aggregates to assist scientists with disease model development and accelerate neurodegenerative disease drug discovery.

Tau Pre-formed Fibrils (PFFs), Filaments & Monomers 

 

 

Tau Pre-formed Fibrils (PFFs)

StressMarq manufactures active tau protein constructs to help researchers study tau aggregation, a hallmark of neurodegenerative diseases including Alzheimer’s. StressMarq was the first commercial source of active tau pre-formed fibrils (PFFs) for neuroscience research, and offers the broadest range of tau constructs to researchers worldwide.

The process of tau aggregation can be seeded by active tau PFFs, which recruit monomers to form larger tau fibrils. This has been demonstrated in thioflavin T assays where an increase in fluorescence – indicative of tau fibrillization – is seen when active tau PFFs are combined with active tau monomers. Some tau PFFs have been injected into P301L mice, where they seed tau aggregation and induce tau pathology in the hippocampus.


Product List | PFFs

Human Tau-441 (2N4R) Wild-Type Pre-formed Fibrils, catalog# SPR-480
Human Tau-441 (2N4R) Wild-Type Pre-formed Fibrils (Baculovirus/Sf9) , catalog# SPR-498 New!
Human Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils, catalog# SPR-329
Human Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils: ATTO 488 conjugated, catalog# SPR-329-A488
Human Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils (Baculovirus/Sf9) , catalog# SPR-471
Human Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils (CHO-expressed), catalog# SPR-516 New!
Human Tau-441 (2N4R) P301S Mutant Filaments, catalog# SPR-463
Mouse Tau-430 (2N4R) P290S Mutant Pre-formed Fibrils, catalog# SPR-475
Human Tau (K18) P301L Mutant Pre-formed Fibrils, catalog# SPR-330
Human Tau (K18) Delta K280 Mutant Pre-formed Fibrils, catalog# SPR-477
Human Tau-352 (fetal 0N3R) Wild-Type Pre-formed Fibrils, catalog# SPR-491
Human Tau-381 (1N3R) Wild-Type Pre-formed Fibrils, catalog# SPR-514 New!
Human Tau-383 (0N4R) Wild-Type Pre-formed Fibrils, catalog# SPR-510 New!
Human Tau-412 (1N4R) Wild-Type Pre-formed Fibrils, catalog# SPR-512 New!
Human Tau dGAE, C322A (AA297-391) Pre-formed Fibrils, catalog# SPR-462
Human Tau dGAE (AA297-391) Pre-formed Fibrils, catalog# SPR-461
Human Tau dGAE (AA297-391) AD-mimic Pre-formed Fibrils, catalog# SPR-502 New!

Tau & Alpha Synuclein Co-Polymer Fibrils (Mixed Fibrils/PFFs) 

StressMarq’s co-polymer fibrils are developed by co-incubating monomers together to form fibrils that contain both tau and alpha synuclein proteins within a single fibril. These co-polymer fibrils have been demonstrated to seed fibril formation of both tau monomers and of a mixture of alpha synuclein and tau monomers.


Product List | Co-Polymer Fibrils

Human Tau-352 (fetal 0N3R) & Human Alpha Synuclein Co-Polymer Fibrils, catalog# SPR-494
Human Tau-441 (2N4R) & Human Alpha Synuclein Co-Polymer Fibrils, catalog# SPR-495

Tau Monomers

StressMarq’s tau monomers are capable of aggregation. They do not show neurodegenerative activity.

Product List | Monomers

Human Tau-441 (2N4R) Wild-Type Monomers, catalog# SPR-479
Human Tau-441 (2N4R) Wild-Type Monomers (Baculovirus/Sf9), catalog# SPR-496 New!
Human Tau-441 (2N4R) P301S Mutant Monomers, catalog# SPR-327
Human Tau-441 (2N4R) P301S Mutant Monomers (Baculovirus/Sf9) , catalog# SPR-473
Human Tau-441 (2N4R) P301S Mutant Monomers (CHO-expressed, N-glycosylated), catalog# SPR-515 New!
Mouse Tau-430 (2N4R) P290S Mutant Monomers, catalog# SPR-474
Human Tau (K18) P301L Mutant Monomers, catalog# SPR-328
Human Tau (K18) P301L Mutant Monomers, catalog# SPR-328
Human Tau (K18) Delta K280 Mutant Monomers, catalog# SPR-476
Human Tau-352 (fetal 0N3R) Wild-Type Monomers, catalog# SPR-490
Human Tau-381 (1N3R) Wild-Type Monomers, catalog# SPR-513 New!
Human Tau-383 (0N4R) Wild-Type Monomers, catalog# SPR-509 New!
Human Tau-412 (1N4R) Wild-Type Monomers, catalog# SPR-511 New!
Human Tau dGAE, C322A (AA297-391) Monomers, catalog# SPR-445
Human Tau dGAE Truncated Fragment (AA297-391) Monomers, catalog# SPR-444
Human Tau dGAE (AA297-391) Monomers, catalog# SPR-501 New!

 

Selected Scientific & Product Information

SPR-330_Tau Preformed Fibrils Protein IHC-1

Immunohistochemistry analysis of P301L mouse hippocampus injected with K18 P301L tau PFFs (SPR-330) shows seeding of tau pathology at injection site nine weeks post-injection. AT8 (pSer202/pThr205) tau antibody shows tangle-like inclusions. Inset: negative control. Experiments performed at reMYND N.V.

Monomers and PFFs (fibrils) are available in a variety of lengths. Tau-441 has a molecular weight of approximately 46 kDa, whereas K18 tau has a molecular weight of approximately 15 kDa.  dGAE is a fragment of the tau protein consisting of amino acids 297-391. It is one of the core PHF subunits,1 includes both microtubule-binding domains and proline-rich regions, and assembles into PHF-like fibrils in vitro without additives or templates.

Tau proteins are available as wild-type or with a variety of mutations. P301S and P301L mutations occur in exon 10 and are associated with frontotemporal dementia. The P301S mutation reduces tau’s ability to assemble microtubules, and the P301L mutation promotes beta-sheet formation and the formation of PHFs. Both P301S and P301L mutant transgenic mouse models are used in tau research. The K280 deletion mutation is also associated with frontotemporal dementia and promotes fibrillization into paired helical filaments (PHFs) in the absence of heparin and other inducers. The C322A mutation also increases tau’s ability to form PHFs.2

PFFs induce tau aggregation; full-length PFFs may be more effective in seeding fibrillization, but a combination of both can be particularly toxic to neurons. Most tau constructs are fibrillized using a heparin scaffold; soluble tau filaments are fibrillized using a linear anionic scaffold. dGAE tau and K18 K280 deletion tau both fibrillize without scaffolds.

StressMarq offers certain Tau PFFs expressed in baculovirus. Baculovirus tau PFFs are post-translationally modified and fibrillize without a scaffold.

1. Novak, M. et al. (1993) EMBO J. 12, 365-370.
2. Al-Hilaly, Y.K. et al. (2017) J. Mol. Biol. 429(23):3650-3665.

 

TEM of active recombinant Tau441 (2N4R), P301S mutant Preformed Fibrils (PFFs)

TEM of active recombinant Tau-441 (2N4R), P301S mutant pre-formed fibrils (PFFs) (SPR-329).

TEM of active recombinant Tau (K18), P301L mutant Preformed Fibrils (PFFs)

TEM of active recombinant Tau (K18), P301L mutant pre-formed fibrils (PFFs) (SPR-330).

TEM of Human Recombinant Tau Protein Filaments (SPR-463)

TEM of recombinant Tau Filaments (SPR-463).

 

 

 

 

 

 

 

 

Scientific Publications Using StressMarq’s Tau Proteins

Most Recent Tau Citations

  • ApoE4 expression disrupts tau uptake, trafficking, and clearance in astrocytes. Eisenbaum, M. et al. Glia 2024
  • APOE3ch alters microglial spread and suppresses Aβ-induced seeding and spread. Chen, Y. et al. Cell 2023
    • Catalog# SPR-329: Human Recombinant Tau-441 (2N4R) P301S Mutant Pre-formed Fibrils
  • In vivo near-infrared fluorescence imaging selective for soluble amyloid beta aggregates using y-shaped BODIPY derivative. Akasaka, T. et al. Journal of Medicinal Chemistry 2023
    • Catalog# SPR-477: Human Recombinant Tau (K18) Delta K280 Mutant Pre-formed Fibrils

Supplemental Learning Materials

Technical Support Resources

Selected Media from the StressMarq YouTube Channel 

Selected Articles from the StressMarq Blog


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