Antibodies - Heat Shock Proteins, Oxydative Stress, Apoptosis, Cell Signaling

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Product Name: Nitrotyrosine
Catalog #: SMC-154D
Alternative Names: N/A
Clone Number: 39B6
Immunogen: Hybridoma line 39B6
Accession Number: N/A
SwissProt: N/A
Applications: WB, ELISA, IHC, IP
Host Species: Mouse
Isotype: IgG2a
Species Reactivity: Human, Mouse, Rat, Dog
Recommended Dilutions: 0.7μg/ml was sufficient for detection of 5µg SIN-1 treated BSA by Western Blot
Form: Protein G purified
Storage Buffer: PBS, 50% glycerol
Concentration: 1mg/mL
Background Info: Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.
Conjugate: N/A
Package Size: 100ug
Storage Temp: -20°C
Shipping Temp: Blue Ice or 4°C
Datasheet: SMC 154 Nitrotyrosine Cell Signaling
Research Area: Cell Signaling, Oxidative Stress, Post-translational Modifications
Certificate of Analysis: 0.7μg/ml of SMC-154 was sufficient for detection of 5μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Price: $297.00 USD Add to Cart

Nitrotyrosine visualized using SMC-154, tested in rat liver tissues.

Nitrotyrosine visualized using SMC-154, tested on Bouin's fixed paraffin-embedded backskin sections of transgenic mice.
Courtesy of Dr. Turksen, Ottawa Hospital Research Institute, Canada.

Research Background: Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosino-philperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer’s disease, Parkinson’s disease and amyotrophic lateral sclerosis (4).
References: 1. Girault I. et al. (2001). Free Radical Biology and Medicine, 31 (11): 1375-1387.
2. Gow AJ, Farkouh CR, Munson DA, Posencheq MA, and Ischiropoulos H. (2004). Am J Physiol Lung Cell Mol Physiol. 287(2): L262-8.
3. Takemoto K. et al (2007). Acta Med Okayama 61(1): 17-30.
4. Reynolds MR. et al. (2006) J Nerosci. 26(42): 10636-45.
5. Pfister H., et al. (2002) Vet Pathol. 39: 190-199.
6. Khan J. et al. (1998) Biochem J. 330(2): 795-801.
Cited References: Hyun-Seok Kim, Krish Patel, Kristi Muldoon-Jacobs, Kheem S. Bisht, Nukhet Aykin-Burns, J. Daniel Pennington, Riet van der Meer, Phuongmai Nguyen, Jason Savage, Kjerstin M. Owens, Athanassios Vassilopoulos, Ozkan Ozden, Seong-Hoon Park, Keshav K. Singh, Sarki A. Abdulkadir, Douglas R. Spitz, Chu-Xia Dengsend email, David Giuss. Cancer Cell, Volume 17, Issue 1, 41-52, 19 January 2010

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