Antibodies - Heat Shock Proteins, Oxydative Stress, Apoptosis, Cell Signaling

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Product Name: GRP78
Catalog #: SPC-107B
Alternative Names: BIP, Grp78, HspA5, MIF2, immunoglobulin heavy chain binding protein
Clone Number: N/A
Immunogen: Rat GRP78 (Bip) sythetic peptide conjugated to KLH
Accession Number: NP_005338.1
Gene ID: 3309
SwissProt: P11021
Applications: WB
Host Species: Rabbit
Isotype: N/A
Species Reactivity: Human, Mouse, Rat, Cow, Fungus (Cryphonectria parasitica), Hamster, Monkey, Rabbit, Xenopus
Recommended Dilutions: 1:2000 (WB)
Form: Rabbit antiserum
Storage Buffer: Rabbit antiserum
Concentration: N/A
Background Info: Detects a protein with a mass of ~78kDa corresponding to Grp78.
Conjugate: N/A
Package Size: 200ul
Storage Temp: -20°C
Shipping Temp: Blue Ice or 4°C
Datasheet: SPC 107 Glucose Regulated Protein 78 (Grp78) (BIP)
Research Area: Chaperones, Heat Shock, Trafficking
Certificate of Analysis: 0.5 μg/ml of SPC-107 was sufficient for detection of Grp78 in 10ug of rat tissue lysate by ECL immunoblot analysis.
Price: $299.00 USD Add to Cart

Western blot analysis of GRP78 in (from L-R): Recombinant protein, human, dog and mouse tissues using a 1:1000 dilution of SPC-107.

Western blot analysis of GRP78 in rat tissues, using a 1:1000 dilution of SPC-107.

Research Background: GRP78 is a ubiquitously expressed, 78-kDa glucose regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).
References: 1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83.
2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555.
3. Luo S., et al (2006) 26 (15): 5688-97.
4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314.
5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336.
6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949.
7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.

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