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Product Name
DNA Damage (8-OHdG) ELISA Kit
Catalog #
SKT-120-96S
Size
96-well
Price
$391.00 USD
Bulk Quote
Alternate Product Sizes
SKT-120-480S
Type
ELISA
Description
Colorimetric detection of 8-hydroxy-2-deoxy Guanosine
Research Area
Oxidative Stress
Alternative Names
8-OH-dG, 8OHG, 80G, 8 hydroxyguanine, DNA Damage ELISA, 8OHdG ELISA, 8-ohdg elisa kit, Enzyme-Linked Immunosorbent Assay, 8-OHdG
Species Reactivity
ALL
Sample Type
Urine, Cell Lysates, Plasma, Sample Matrices
Sensitivity
0.59 ng/mL
Assay Range
0.94 - 60 ng/mL
Incubation Time
1 hour
Storage Temp
4°C and -20°C
Shipping Temp
Blue Ice, 4°C
Other Relevant Information
Samples/Kit: 39 in duplicate



DNA Damage ELISA Kit Catalog # SKT-120 MSDS

                                


Catalog Number Item Quantity/ Size
(96 wells)
SKC-120A 8-hydroxy-2-deoxy Guanosine : BSA Coated Plate  1 Plate
SKC-120C 8-hydroxy-2-deoxy Guanosine Standard  1 vial/ 100uL
SKC-120F 8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody  1 vial/75uL
SKC-0001 Sample and Standard Diluent  1 vial/50mL
SKC-0002 8-hydroxy-2-deoxy Guanosine Antibody Diluent  1 vial/13mL
SKC-0003 Wash Buffer Concentrate  1 vial/50mL
SKC-0004 TMB Substrate  1 vial/13mL
SKC-0005 Stop Solution  1 vial/13mL
SKC-0009 Plate Cover  2 covers

Typical Standard Curve for the DNA Damage (8-OHdG) ELISA kit (Enzyme-Linked Immunosorbent Assay) StressXpress® - SKT-120.
Assay Type: Competitive ELISA.
Detection Method: Colorimetric Assay.
Assay Range: 0.94 - 60 ng/ml.



Excel Analysis Tools

8-OHdG ELISA Kit Calculations Worksheet (for use with duplicate and triplicate samples)

 


Discovering biomarkers for antidepressant response: protocol from the Canadian biomarker integration network in depression (CAN-BIND) and clinical characteristics of the first patient cohort.
BMC Psychiatry. 16(1):105. 2016. Lam, R.W. et al.
PubMed ID: 27084692.     Reactivity: Human.     Application: ELISA.

Podocyte-specific Nox4 deletion affords renoprotection in a mouse model of diabetic nephropathy.
Diabetologia. [Epub ahead of print]. 2015. Jha, J.C. et al.
PubMed ID:26508318    Reactivity:Mouse    Applications:EIA

Does chronic raise of metal ion levels induce oxidative DNA damage and hypoxia-like response in patients with metal-on-metal hip resurfacing?
J Biomed Mater Res B Appl Biomater. [Epub ahead of print]. 2015. Savarino, L. et al.
PubMed ID: 26477446    Reactivity: Human    Applications: EIA

Higher urinary Levels of 8-hydroxy-2'-deoxyguanosine are associated with a worse RANKL/OPG ratio in postmenopausal women with osteopenia. (View PDF)
Oxidative Medicine and Cellular Longevity. [Epub ahead of print]. 2015. Cervellati, C. et al.
PubMed ID: N/A      Reactivity: Human    Applications: EIA

Turmerone enriched standardized Curcuma longa extract alleviates LPS induced inflammation and cytokine production by regulating TLR4–IRAK1–ROS–MAPK–NFκB axis.
J. Functional Foods. 16 (2015): 152–163. 2015. Rana, M. et al.
PubMed ID: N/A     Reactivity: Mouse     Applications: EIA

Synthesis, characterization and biological activity of some unsymmetrical Schiff base transition metal complexes.
Drug Chem Toxicol. [Epub ahead of print]. 2015. Esmadi, F.T. et al.
PubMed ID: 25791998     Reactivity: Human     Applications: ELISA

Immediate and delayed effects of growth conditions on ageing parameters in nestling zebra finches.
The Journal of Experimental Biology. [Epub ahead of print]. 2015. Reichert, S. et al.
PubMed ID: 25524985     Reactivity: Zebra finch      Applications: EIA

Late-intervention study with ebselen in an experimental model of type 1 diabetic nephropathy.
Free Radic Res. [Epub ahead of print]. 2014. Tan, S.M., Sharma, A., Stefanovic, N. and de Haan, J.B.
PubMed ID: 25465090     Reactivity: Mouse     Applications: EIA

A Derivative of Bardoxolone methyl, dh404, in an inverse dose-dependent manner, lessens diabetes-associated atherosclerosis and improves diabetic kidney disease.
Diabetes. 63(9):3091-103. 2014. Tan, S. M., et al.
PubMed ID: 24740568     Reactivity: Mouse     Applications: EIA
 
Elevation impacts the balance between growth and oxidative stress in coal tits.
Oecologia. 175(3):791-800. 2014. Stier, A., et al.
PubMed ID: 24805201     Reactivity: Bird (coal tits)     Applications: EIA

Effect of Therapeutic Hypothermia on DNA Damage and Neurodevelopmental Outcome Among Term Neonates with Perinatal Asphyxia: A Randomized Controlled Trial.
J Trop Pediatr.  60(2):134-40. 2014. Gane, B.D., et al.
PubMed ID: 24343823     Reactivity: Human     Applications: ELISA

Evaluation of vitamin B12 effects on DNA damage induced by paclitaxel.
Drug Chem Toxicol. 37(3):276-80. 2014. Alzoubi, K., Khabour O., Khader M., Mhaidat N. and Al-Azzam, S.
PubMed ID: 24215581     Reactivity: Human     Applications: ELISA

The Modified Selenenyl Amide, M-hydroxy Ebselen, Attenuates Diabetic Nephropathy and Diabetes-Associated Atherosclerosis in ApoE/GPx1 Double Knockout Mice. (View PDF)
PLoS ONE 8(7): e69193.2013. Tan, S.M., et al.
PubMed ID: 23874911     Reactivity: Mouse     Applications: EIA
 
Oxidative Damage of DNA Confers Resistance to Cytosolic Nuclease TREX1 Degradation and Potentiates STING-Dependent Immune Sensing.
Immunity. 39(3):482-95. 2013. Gehrke, N. et al.
PubMed ID: 23993650     Reactivity: Mouse     Applications: EIA
 
Disruption of pro-oxidant and antioxidant systems with elevated expression of the ubiquitin proteosome system in the cachectic heart muscle of nude mice. (View PDF)
J. Cachexia, Sarcopenia and Muscle. 4(4):287-93. 2013. Hinch, E. C. A., Sullivan-Gunn, M. J., Vaughan V. C., McGlynn M. A., Lewandowski, Paul A.
PubMed ID: 24030522     Reactivity: Mouse     Applications: ELISA
 
TiO2 Nanoparticle Exposure and Illumination during Zebrafish Development: Mortality at Parts per Billion Concentrations.
Environ. Sci. Technol.  47 (9), 4726-4733. 2013. Bar-Ilan, O. et al.
PubMed ID: 23510150     Reactivity: Zebrafish     Applications: ELISA
 
Light-induced cell detachment for cell sheet technology.
Biomaterials. 34 (1), 11-18. 2013. Hong, Y. et al.
PubMed ID: 23069710     Reactivity: Mouse     Applications: EIA
 
Grasshoppers' adaptation to elevated radioactivity in the Chernobyl exclusion zone.
Master Thesis in Environmental and Molecular Biology. ENSPAC and NSM, Roskilde University, Denmark. 2013. Mortensen, L.H.
PubMed ID: N/A     Reactivity: Grasshopper     Applications: EIA
 
Consumption of a low glycaemic index diet in late life extends lifespan of Balb/c mice with differential effects on DNA damage. (View PDF)
Longevity & Healthspan. 2(1):4. 2013. Nankervis, S.A., Mitchell, J.M., Charchar, F.J., McGlynn, M.A. and Lewandowski, P.A.
PubMed ID: 24472560     Reactivity: Mouse     Applications: ELISA
 
Poor sleep in PCOS; is melatonin the culprit? (View PDF)
Hum. Reprod. 28 (5), 1348-1353. 2013. Shreeve, N. et al.
PubMed ID: 23438443     Reactivity: Human     Applications: ELISA
 
Differential effects of docosahexanoic acid (DHA) on preterm and term placental pro-oxidant/anti-oxidant balance. (View PDF)
Reproduction. 146(3):243-51. 2013. Stark, M. et al.
PubMed ID: 23813449     Reactivity: Human     Applications: ELISA
 
Protective Effects of Salvianolic Acid B on Schwann Cells Apoptosis Induced by High Glucose.
Neurochemical Research. 37 (5), 996-1010. 2012. Sun, L. et al.
PubMed ID: 22252725     Reactivity: Mouse     Applications: ELISA
 
The protective effect of Alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose.
Biochemical Pharmacology. 84 (7), 961-973. 2012. Sun, L. et al.
PubMed ID: 22796564     Reactivity: Mouse     Applications: ELISA
 
Eicosapentaenoic Acid and Oxypurinol in the Treatment of Muscle Wasting in a Mouse Model of Cancer Cachexia. (View PDF)
PLoS ONE 7(9): e45900. 2012. Vaughan VC, Sullivan-Gunn M, Hinch E, Martin P, Lewandowski PA
PubMed ID: 23029301     Reactivity: Mouse     Applications: ELISA
 
Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress.
Neural Regeneration Research. 7 (33), 2583-2591. 2012. Wu, Y., Xue, B., Li, X. and Liu, H.
PubMed ID: N/A     Reactivity: Mouse     Applications: ELISA
 
Ginsenoside Rb1 relieves glucose fluctuation induced oxidative stress and apoptosis in Schwann cells.
Neural Regeneration Research. 2012 (30), 2340-2346. 2012. Xue, B. et al.
PubMed ID: N/A     Reactivity: Mouse     Applications: ELISA
 
Titanium dioxide nanoparticles produce phototoxicity in the developing zebrafish.
Nanotoxicology. 6 (6), 670-679. 2012. Bar-Ilan, O. et al.
PubMed ID: 21830861     Reactivity: Zebrafish     Applications: ELISA
 
Inhibitory effects of Salvianolic acid B on apoptosis of Schwann cells and its mechanism induced by intermittent high glucose.
Life Sciences. 90 (3-4), 99-108. 2012. Sun, L. et al.
PubMed ID: 22036624     Reactivity: Mouse     Applications: ELISA
 
Evaluation of vitamin B12 effects on DNA damage induced by pioglitazone.
Mutat Res. 748 (1-2), 48-51. 2012. Alzoubi, K., Khabour, O., Hussain, N., Al-azzam, S. and Mhaidat, N.
PubMed ID: 22790087     Reactivity: Human     Applications: EIA
 
Oxidative Stress in HPV-Driven Viral Carcinogenesis: Redox Proteomics Analysis of HPV-16 Dysplastic and Neoplastic Tissues. (View PDF)
PLoS ONE. 7(3): e34366. 2012. De Marco, F. et al.
PubMed ID: 22470562     Reactivity: Human     Applications: EIA
 
The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH. (View PDF)
Comparative Hepatology. 10(1):10. 2011. Janevski, M., Antonas, K.N., Sullivan-Gunn, M.J., McGlynn, M.A. and Lewandowski, P.A.
PubMed ID: 22081873     Reactivity: Rat     Applications: ELISA
 
Reduction of Oxidative Damage Reflects a Better Kidney Transplantation Outcome. 
Am J Nephrol. 34, 496-504. 2011. La Manna, G. et al. 
PubMed ID: 22041478     Reactivity: Human     Applications: ELISA
 
Transgenic Mice Expressing Cyclooxygenase-2 in Hepatocytes Reveal a Minor Contribution of This Enzyme to Chemical Hepatocarcinogenesis. (View PDF)
The American Journal of Pathology. 178 (3), 1361-1373. 2011. Izquierdo, C.L. et al.
PubMed ID: 21356386     Reactivity: Mouse     Applications: EIA
 
A qPCR-based assay to quantify oxidized guanine and other FPG-sensitive base lesions within telomeric DNA.
Biotechniques. 51 (6), 403-411. 2011. O'Callaghan, N., Baack, N., Sharif, R. and French, M.
PubMed ID: 22150331     Reactivity: Human     Applications: ELISA
 
Integrated exposure assessment of sewage workers to genotoxicants: an urinary biomarker approach and oxidative stress evaluation.
Environmental Health. 10 (23). 2011. Zabadi, H.A. et al.
PubMed ID: 21435260     Reactivity: Human     Applications: EIA
Research Background
8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA (see Figure 1, on page 6) by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension (5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OHdG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OHdG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OHdG than plasma. Urinary levels of 8-OHdG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OHdG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
References
1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) Interference in enzyme immunoassays. J Clin Immunoassay 15: 116-120.
2. Pradelles P., Grassi J., Maclouf J. (1990) Enzyme immunoassays ofeicosanoids using acetylcholinesterase. Methods Enzymol 187: 24-34.
3. Maclouf J., Grassi J., Pradelles P. (1987) Development of enzyme-immunoassay techniques for the measurement of eicosanoids.
4. Lin H., et al. (2004) A high-throughput and sensitive methodologyfor the quantification of urinary 8-hydroxy-2’-deoxyguanosine:Measurement with gas chromatography-mass spectrometry after singlesolid-phase extraction. Biochem J 380: 541-548.
5. Bogdanov M.B., et al. (1999) A carbon column-based liquidchromatography electrochemical approach to routine8-hydroxy-2’-deoxyguanosine measurements in urine and other biologicmatrices: A one-year evaluation of methods. Free Radic Biol Med27(5/6): 647-666.
6. Lee J., et al. (2005) Carvedilol reduces plasma8-hydroxy-2’-deoxyguanosine in mild to moderate hypertension. A pilotstudy. Hypertension 45: 986-990.
7. Leinonen, J., et al. (1997) New biomarker evidence of oxidative DNAdamage in patients with non-insulin-dependent diabetes mellitus. FEBSLett 417: 150-152.
8. Endo K., et al. (2006) Probucol and atorvastatin decrease urinary8-hydroxy-2’-deoxyguanosine in patients with diabetes andhypercholesterolemia. Journal of Atherosclerosis and Thrombosis 13:68-75.
9. Kuo H., et al. (2007) Urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG)and genetic polymorphisms in breast cancer patients. Mutat Res 631:62-68.
10. Shen J., et al. (2007) 8-hydroxy-2’-deoxyguanosine (8-OH-dG) as apotential survival biomarker in patients with nonsmall-cell lungcancer. Cancer 109: 574-580.
11. Beckman K.B., Ames B.N. (1997) Oxidative decay of DNA. J Biol Chem 272: 19633-19636.
12. Epe B., et al. (1996) DNA damage by peroxynitrite characterized with DNA repair enzymes. Nucleic Acids Res 24: 4105-4110.
13. Spencer J.P.E., et al. (1995) DNA strand breakage and basemodification induced by hydrogen peroxide treatment of humanrespiratory tract epithelial cells. FEBS Lett 374: 233-236.
14. Floyd R.A. (1990) Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB J 4: 2587-2597.
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