Product Name

DNA Damage, 8-OH-dG StressXpress® EIA Kit

Catalog #

SKT-120-96

Alternative Names

8-OH-dG, 8OHG, 80G, 8 hydroxyguanine

Size

96-well

Type

EIA Kit

Description

8-OHdG detection

Species Reactivity

N/A, 8OHdG

Sample Type

Urine, cell culture, plasma and other sample matrices

Research Area

Oxidative Stress

Incubation Time

18hours

Sensitivity

30pg/mL

Assay Range

100-30pg/mL

Storage Temp

-20°C

Shipping Temp

Blue Ice, 4°C

Price

$380.00 USD Add to Cart Bulk Quote

8-OH-2-deoxy Guanosine EIA Double Worksheets (for use with duplicate samples)

8-OH-2-deoxy Guanosine EIA Triple Worksheets (for use with triplicate samples)

Research Background

8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA (see Figure 1, on page 6) by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).

References

1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) Interference in enzyme immunoassays. J Clin Immunoassay 15: 116-120.
2. Pradelles P., Grassi J., Maclouf J. (1990) Enzyme immunoassays ofeicosanoids using acetylcholinesterase. Methods Enzymol 187: 24-34.
3. Maclouf J., Grassi J., Pradelles P. (1987) Development of enzyme-immunoassay techniques for the measurement of eicosanoids.
4. Lin H., et al. (2004) A high-throughput and sensitive methodologyfor the quantification of urinary 8-hydroxy-2’-deoxyguanosine:Measurement with gas chromatography-mass spectrometry after singlesolid-phase extraction. Biochem J 380: 541-548.
5. Bogdanov M.B., et al. (1999) A carbon column-based liquidchromatography electrochemical approach to routine8-hydroxy-2’-deoxyguanosine measurements in urine and other biologicmatrices: A one-year evaluation of methods. Free Radic Biol Med27(5/6): 647-666.
6. Lee J., et al. (2005) Carvedilol reduces plasma8-hydroxy-2’-deoxyguanosine in mild to moderate hypertension. A pilotstudy. Hypertension 45: 986-990.
7. Leinonen, J., et al. (1997) New biomarker evidence of oxidative DNAdamage in patients with non-insulin-dependent diabetes mellitus. FEBSLett 417: 150-152.
8. Endo K., et al. (2006) Probucol and atorvastatin decrease urinary8-hydroxy-2’-deoxyguanosine in patients with diabetes andhypercholesterolemia. Journal of Atherosclerosis and Thrombosis 13:68-75.
9. Kuo H., et al. (2007) Urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG)and genetic polymorphisms in breast cancer patients. Mutat Res 631:62-68.
10. Shen J., et al. (2007) 8-hydroxy-2’-deoxyguanosine (8-OH-dG) as apotential survival biomarker in patients with nonsmall-cell lungcancer. Cancer 109: 574-580.
11. Beckman K.B., Ames B.N. (1997) Oxidative decay of DNA. J Biol Chem 272: 19633-19636.
12. Epe B., et al. (1996) DNA damage by peroxynitrite characterized with DNA repair enzymes. Nucleic Acids Res 24: 4105-4110.
13. Spencer J.P.E., et al. (1995) DNA strand breakage and basemodification induced by hydrogen peroxide treatment of humanrespiratory tract epithelial cells. FEBS Lett 374: 233-236.
14. Floyd R.A. (1990) Role of oxygen free radicals in carcinogenesis and brain ischemia. FASEB J 4: 2587-2597.

Cited References

1.  Ofek Bar-Ilan, Kacie M. Louis, Sarah P. Yang, Joel A. Pedersen, Robert J. Hamers, Richard E. Peterson, & Warren Heideman. Titanium dioxide nanoparticles produce phototoxicity in the developing zebrafish. August 10, 2011. doi:10.3109/17435390.2011.604438.

2.  Gaetano La Mannaa, Nicole Lancia, Elena Della Bellaa, Giorgia Comaia, Maria Laura Cappuccillia, Katia Nisia, Paola Todeschinia, Elisa Carrettab, Maria Piera Scolaria, Sergio Stefonia. Reduction of Oxidative Damage Reflects a Better Kidney Transplantation Outcome. Am J Nephrol 2011;34:496-504. DOI: 10.1159/000329320

3.  Lian-Qing Suna, Bing Xuea, Xiao-Jin Lia, Xuan Wanga, Ling Qua, Ting-Ting Zhanga, Jue Zhaoa, Bao-An Wanga, Xiao-Man Zoua, Yi-Ming Mua, Ju-Ming Lu. Inhibitory effects of Salvianolic acid B on apoptosis of Schwann cells and its mechanism induced by intermittent high glucose. Life Sciences. doi:10.1016/j.lfs.2011.10.001.

4.  Mile Janevski, Kiriakos N Antonas, Melanie J Sullivan-Gunn, Maree A McGlynn, Paul A Lewandowski. The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH. Comparative Hepatology 2011, 10:10 doi:10.1186/1476-5926-10-10

5.  Nathan O'Callaghan, Natalie Baack, Razinah Sharif, and Michael Fenech.  A qPCR-based assay to quantify oxidized guanine and other FPG-sensitive base lesions within telomeric DNA. BioTechniques, Vol. 51, No. 6, December 2011, pp. 403–412

6.  Lian-Qing Sun, Jue Zhao, Ting–Ting Zhang, Ling Qu, Xuan Wang, Bing Xue, Xiao-Jin Li, Yi-Ming Mu and Ju-Ming Lu. Protective Effects of Salvianolic Acid B on Schwann Cells Apoptosis Induced by High Glucose. Neurochemical Research; DOI: 10.1007/s11064-011-0695-8

7.  Prinsloo, E., Kramer, A. H., Edkins, A. L. and Blatch, G. L. (2012), STAT3 interacts directly with Hsp90. IUBMB Life. doi: 10.1002/iub.607

8.  De Marco F, Bucaj E, Foppoli C, Fiorini A, Blarzino C, et al. (2012) Oxidative Stress in HPV-Driven Viral Carcinogenesis: Redox Proteomics Analysis of HPV-16 Dysplastic and Neoplastic Tissues. PLoS ONE 7(3): e34366. doi:10.1371/journal.pone.0034366