StressXpress®
Alpha B Crystallin ELISA Kit

Colorimetric detection of Alpha B Crystallin

Catalog No. SKT-123

5 out of 5 based on 1 customer rating
Species Reactivity Hu
Sample Types Cell lysates, Serum, Tissue
SKU: SKT-123 Categories: ,

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SKT-123_Alpha_B_Crystallin_ELISA_Kit_Standard_Curve.png
Product Name Alpha B Crystallin ELISA Kit
Description

Colorimetric detection of Alpha B Crystallin

Species Reactivity Human
Platform Microplate
Sample Types Cell lysates, Serum, Tissue
Detection Method Colorimetric Assay
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Utility ELISA kit used to quantitate alpha B crystallin concentration in samples.
Sensitivity 0.009 ng/ml
Assay Range 0.352 - 22.5 ng/ml
Incubation Time 30 minutes
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet Lot No. SA222909 , Kit Booklet Lot No. SA388800 , Kit booklet Lot No. SA287484 , Kit Booklet , MSDS
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Assay Overview 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Kit Overview
Component No.ItemQuantity / Size
SKC-123A Anti-Alpha B Crystallin Immunoassay Plate 1 Plate
SKC-123B 5X Alpha B Crystallin Extraction Reagent 1 bottle/10 ml
SKC-123C Recombinant Alpha B Crystallin Standard 2 vials
SKC-123D Standard and Sample Diluent 1 bottle/ 50 ml
SKC-123E 10X Wash Buffer Concentrate 1 bottle/100 ml
SKC-123F Anti-Alpha B Crystallin Biotinylated Antibody Concentrate 1 vial/150 µl
SKC-123G Anti-Alpha B Crystallin Biotinylated Antibody Diluent 1 bottle/ 13 ml
SKC-123H Streptavidin: HRP Concentrate 1 vial/150 µl
SKC-123I Streptavidin: HRP Diluent 1 bottle/ 13 ml
SKC-123J TMB Substrate 1 bottle/ 13 ml
SKC-123K Stop Solution 1 bottle/ 13 ml
Cite This Product Alpha B Crystallin ELISA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-123)

Biological Description

Alternative Names AACRYA ELISA Kit, CRYA2 ELISA Kit, CRYAB ELISA Kit, CTPP2 ELISA Kit, HSPB5 ELISA Kit, NY Ren 27 antigen ELISA Kit, alpha crystallin B ELISA Kit
Scientific Background The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with HSP25 and HSP27 (1). Mammalian lens cystallins are divided into alpha, beta and gamma families. alpha and beta families are further divided into acidic and basic groups (alpha-A and alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance (2). In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA (3, 4, 5, 6, and 7). Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases (8). Specifically, alpha-B cystallin is found in many cells and organs outside the lens, and alpha B is overexpressed in several neurological disorders and in cell lines under stress conditions (9).
References 1. Merck K.B. et al. (1993) J Biol Chem. 268: 1046-1052.
2. Horwitz J. (1992) Proc Natl Acad Sci USA 89(21): 10449-10453.
3. Cobb B.A. and Petrash J.M. (2002) Biochemistry. 41: 483-490
4. Horwitz J. (2003) Exp Eye Res. 76: 145-153.
5. Bullard B. et al. (2004) J Biol Chem. 279: 7917-7924.
6. Gangalum R.K., Schibler M.J. and Bhat S.P. (2004) J Biol Chem. 279: 43374.43377.
7. Maddala R. and Rao V.P. (2005) Exp Cell Res. 306: 203-215.
8. Yaung J., et al. (2007) Molecular Vision 13: 566-577.
9. Head M.W. et al. (2000) Neuropathol Appl Neurobiol. 26: 304-312.

Product Images

<p>Typical Standard Curve for the Alpha B Crystallin ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-123. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.352 – 22.5 ng/mL.</p>

Typical Standard Curve for the Alpha B Crystallin ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-123. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.352 – 22.5 ng/mL.

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