StressXpress®
DUB Activity Kit

Fluorometric detection of DUB activity

Catalog No. SKT-136

0 out of 5 based on 0 customer ratings
Species Reactivity ALL
Sample Types Purified Proteins
SKU: SKT-136 Categories: ,

This product is currently out of stock and unavailable.

SKT-136_DUB_Activity_Kit_Enzyme_Activity_Graph.png
Graph of the AMC Typical Standard Curve for the DUB Activity Kit StressXpress - SKT-136
Product Name DUB Activity Kit
Description

Fluorometric detection of DUB activity

Species Reactivity Species Independent
Platform Microplate
Sample Types Purified Proteins
Detection Method Fluorometric Assay
Assay Type Continuous Kinetic Assay
Utility Activity kit used to screen potential inhibitors and activators against DUB enzymes, and to assess and evaluate performance of DUB activity in purified protein samples. It can also be used to optimize assays for specific DUBs to facilitate their use in HTS, and demonsrate that novel DUB enzymes have ubiquitin-AMC processing activity.
Incubation Time 30-90 minutes
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet , MSDS
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature -80
Shipping Temperature Dry Ice
Product Type Activity Kits
Assay Overview The DUB Activity kit facilitates the rapid, robust measurement of deubiquitinating enzyme activity in vitro. The kit utilises high purity, fluorogenic substrate ubiquitin-AMC together with suitable calibration standards and controls for the accurate and sensitive assessment of DUB activity. Continuous kinetic or end-point assays can be performed in 96-well plate format for multi-sample analysis. Contains sufficient materials for one full 96-well plate assay set-up to be run.
Kit Overview
Component No.ItemQuantity / Size
SKC-136A 10x StressXpress® DUB assay buffer 1 ml
SKC-136B Control DUB enzyme (USP2 catalytic domain) 10 µl
SKC-136C Ubiquitin-AMC 25 µl
SKC-136D StressXpress® 96 well assay plate 1 plate
SKC-136E StressXpress® AMC standard 17.5 µg
Cite This Product DUB Activity Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-136)

Biological Description

Alternative Names Deubiquitinating enzyme Activity Kit
Research Areas Apoptosis, Autophagy, Cancer, Cardiovascular System, Cell Signaling, Neurodegeneration, Neuroscience, Post-translational Modifications, Ubiquitination
Scientific Background Conjugation of ubiquitin to proteins (ubiquitination) plays a fundamental role in the regulation of cellular function through biological events involving, amongst others, cell cycle, differentiation, immune responses, DNA repair, chromatin structure, and apoptosis. The ubiquitin signaling system includes a large family of cysteine proteases known as deubiquitinating enzymes (DUBs) that are responsible for the removal of ubiquitin from modified proteins. This regulatory process allows optimal levels of cellular ubiquitin to be maintained by recycling ubiquitin attached to inappropriate targets, removing and disassembling polyubiquitin chains, and processing proteins prior to their degradation by the proteasome. DUBs in general exhibit a wide range of substrate, ubiquitination type (mono- or poly-ubiquitination) and polyubiquitin chain linkage specificities and can be partnered with various interacting proteins to facilitate increased diversity in specificity and DUB activation. DUBs have been implicated in a number of human diseases including various forms of cancer and neurodegeneration. As such they are attractive targets for potential therapeutic intervention via the development of suitable inhibitors and modulators.
References 1. Komander, D. & Rape, M. Annual Review of Biochemistry 81, 203–229 (2012).
2. Ciechanover, A. Biochimica et biophysica acta 1824, 3–13 (2012).
3. Clague, M. J., Coulson, J. M. & Urbé, S. Journal of cell science 125, 277–86 (2012).
4. Komander, D., Clague, M. J. & Urbé, S. Nature reviews. Molecular cell biology 10, 550–63 (2009).
5. Fraile, J. M., Quesada, V., Rodríguez, D., Freije, J. M. P. & López-Otín, C. Oncogene 31, 2373–88 (2012).
6. Kessler, B. M. Current opinion in chemical biology 17, 59-65 (2013).
7. Bilguvar, K. et al. Proceedings of the National Academy of Sciences of the United States of America 110, 3489–94 (2013).

Product Images

<p>Continuous kinetic enzyme activity assay showing the time course of control DUB enzyme (USP2 CD) activity with Ub-AMC substrate using StressXpress® DUB Activity Assay kit – Control DUB (10nM) was incubated with 500nM Ub-AMC in DUB assay buffer for 15 minutes at room temperature alongside a substrate only (blank) control reaction. Fluorescence measurements (RFU) were taken at 30 second intervals and plotted vs. time</p>

Continuous kinetic enzyme activity assay showing the time course of control DUB enzyme (USP2 CD) activity with Ub-AMC substrate using StressXpress® DUB Activity Assay kit – Control DUB (10nM) was incubated with 500nM Ub-AMC in DUB assay buffer for 15 minutes at room temperature alongside a substrate only (blank) control reaction. Fluorescence measurements (RFU) were taken at 30 second intervals and plotted vs. time

<p>AMC Standard Curve for StressXpress® DUB Activity Assay Kit. Representative plot of fluorescence measurements (RFU) of serial dilutions of AMC Standard at recommended concentrations.</p>

AMC Standard Curve for StressXpress® DUB Activity Assay Kit. Representative plot of fluorescence measurements (RFU) of serial dilutions of AMC Standard at recommended concentrations.

Reviews

Reviews

There are no reviews yet.

Be the first to review “DUB Activity Kit”

Your email address will not be published. Required fields are marked *