Proteins - Purified Proteins, Heat Shock Proteins, Oxidative Cellular Stress

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Product Name: Aha1 (Recombinant, Yeast, His-tag)
Catalog #: SPR-314C
Alternative Names: Aha1, SHSA1, HspC322, p38
Type: Recombinant Protein
Accession Number: NM_001180522.1
Gene ID: 851800
SwissProt: Q12449
Applications: WB control, functional/activity studies
Conjugate/Tag: His-tag
Form: Affinity Purified
Storage Buffer: 25mM Tris buffer pH72, 150mM NaCl, 0.1mM DTT in 50% glycerol
Concentration: 1.8mg/mL
Certificate of Analysis: This product has been certified >90% pure using SDS PAGE analysis.
Package Size: 200ug
Storage Temp: -20°C
Shipping Temp: Blue Ice or 4°C
Datasheet: SPR 314 Aha1 Heat Shock
Research Area: Heat Shock
Price: $495.00 USD Add to Cart Bulk Quote

SDS PAGE of SPR-314.

ATPase assay of yeast Hsp82 activated by SPR-314. Reaction conditions are 25mM Hepes pH7.2, 2.2mM NaCl, 5mM MgCl2, 2mM ATP, 1mM PEP, 1mM DTT, PK/LDH, 300uM NADH.

Research Background: Aha1 is a member of the Hsp90 cochaperone family, and is thought to stimulate Hsp90 ATPase activity by competing with p23 and other co-chaperones for Hsp90 binding (1, 2). It may affect a step in the endoplasmic reticulum to Golgi trafficking. Aha1 also interacts with HSPCA/Hsp90 and with the cytoplasmic tail of the vesicular stomatistis virus glycoproteins (VSV G) (3). Aha1 is expressed in numerous tissues, including the brain, heart, skeletal muscle, and kidney, and at low levels, the liver and placenta. Aha1 might be a potential therapeutic strategy to increase sensitivity to HSP inhibitors (4).
References: 1. Hainzl O., Lapina M.C., Buchner J., Richter K. (2009) J Biol Chem. Epub.
2. Harst A., Lin H., Obermann W.M. (2005) Biochem J. 387 (pt.3): 789-796.
3. Lotz G.P., Brychzy A., Heinz S., Obermann W.M. (2008) J Cell Sci. 121(pt.5): 717-723.
4. Holmes J.L., Sharp S.Y., Hobbs S., Workman P. (2008) Cancer Res. 68(4): 1188-1197.

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