FCR1

Name: FCR1
SKU: SIH-180
Price: 477.00 USD
Availability: InStock

FRET ratiometric fluorescence-based redox sensor

Catalog No. SIH-180

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CAS No.
Molecular Formula	C34H37N7O6

SKU: SIH-180 Categories: Redox Probe, Small Molecules

Fluorescence lifetimes of the donor in HeLa cells treated with FCR1 (SIH-180), a FRET ratiometric fluorescence-based redox sensor.

Product Name	FCR1
Description	FRET ratiometric fluorescence-based redox sensor
Molecular Formula	C₃₄H₃₇N₇O₆
Molecular Weight	639
Field of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature	-20ºC
Shipping Temperature	Blue Ice or 4ºC
Product Type	Redox Probe
Solubility	Soluble in DMSO
Source	Synthetic
Appearance	Orange Solid
Safety Phrases	Classification: Caution: Substance not yet fully tested. Safety Phrases: S22 - Do not breathe dust S24/25 - Avoid contact with skin and eyes S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection
Cite This Product	FCR1 (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SIH-180)

Biological Description

Alternative Names	7-(Diethylamino)-N-((1r,4r)-4-(2-(10-ethyl-2,4-dioxo-4,10-dihydrobenzo[g]pteridin-3(2H)-yl)acetamido)cyclohexyl)-2-oxo-2H-chromene-3-carboxamide (FCR1)
Research Areas	Cancer, Oxidative Stress
Scientific Background	FCR1 or flavin coumarin redox sensor 1, is a novel ratiometric fluorescent redox sensor utilized in fluorescence lifetime imaging microscopy and flow cytometry. In the oxidized form, excitation of FCR1 at 405nm results in a green fluorescence with max emissions at 525nm. Treatment with a mild reducing agent (including sodium cyanoborohydride, DTT, and glutathione) reduces the flavin thereby decreasing the green fluorescence intensity, and increasing the blue. Re-oxidation can be achieved using mild oxidizing agents. In general, the FCR1 probe can be used to observe changes in oxidative capacity without interference from background effects.
References	1. Kaur A., Haghighatbin M.A., Hogan C.F., and New E.J. (2015) Chem. Commun. Epub.

Product Images

<p>Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Chemical structure and design of FCR1 (SIH-180), showing FRET processes in oxidised form. Inset: photographs of cuvettes of FCR1 in oxidised and reduced forms under 365 nm excitation. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.

<p>Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 µM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Fluorescence behavior of FCR1 (SIH-180) in the oxidized (green) and reduced (blue) forms, using 10 µM. Excitation: 405 nm. Reduced Emission: 475 nm. Oxidized Emission: 520 nm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.

<p>Two photon – confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 µM, 15 min, λex = 820 nm) and (a) N-acetyl cysteine (50 µM, 30 min), (b) vehicle control and (c) H2O2 (50 µM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Two photon – confocal microscopy imaging of HeLa cells treated with FCR1 (SIH-180, 10 µM, 15 min, λex = 820 nm) and (a) N-acetyl cysteine (50 µM, 30 min), (b) vehicle control and (c) H2O2 (50 µM, 30 min) in blue and green channels. The pseudo colour ratio images indicate the ratio of emission intensity in the green channel to blue channel. Scale bar represents 20 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.

<p>Fluorescence lifetimes of the donor (420 – 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Fluorescence lifetimes of the donor (420 – 470 nm) in HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 820 nm) and N-acetyl cysteine, vehicle control and H2O2. Pseudo-colour images represent mean lifetime. Scale bar represents 50 µm. Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.

<p>Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.</p>

Flow cytometric studies of HeLa cells treated with FCR1 (SIH-180, 10 µM, λex = 405 nm) showing the fluorescence ratio (560 / 450 nm) when treated with N-acetyl cysteine (blue), vehicle control (green) and H2O2 (red). Images used with permission from Kaur A, Haghighatbin MA, Hogan CF, New EJ. Chem Commun (Camb). 2015 Jun 16;51(52):10510-3.

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