DNA Damage

8-hydroxy-2-deoxy Guanosine (8-OHdG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress. Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OHdG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension.

DNA damage can be measured by competitive ELISA, which allows for the quantification of 8-OHdG in cell lysates, plasma, sample matrices, and urine. DNA and RNA damage can also be detected with our monoclonal antibody, which is species independent and has been validated for IHC, ICC/IF, ELISA, IP, FCM, and functional assays. Both the monoclonal antibody and ELISA kit have been cited extensively.

  • MOLECULAR SIGNATURE® DNA Damage (8-OHdG) ELISA Kit

    Colorimetric detection of 8-hydroxy-2-deoxy Guanosine

    Catalog No. SKT-120

    5 out of 5 based on 1 customer rating
    Species Reactivity Species Independent
    Sample Types Cell lysates, Plasma, Sample matrices, Urine
    SKU: SKT-120 Categories: ,

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    SKT-120_DNA_Damage_8-OHdG_ELISA_kit_Standard_Curve_Fig6.jpg
    Diagram of the 8-OHdG Competitive ELISA for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Diagram of the Preparation of the 8-OHdG Standards for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Chemical Equation of the Oxidation of Guanosine for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Graph of the Urine Spike Assay for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120