Handling Instructions | Amyloid Beta Proteins

Product Name	Amyloid Beta Proteins (Monomers, Oligomers, Pre-formed Fibrils (PFFs))
Storage Conditions	Monomers, Oligomers, and unsonicated Pre-formed Fibrils (PFFs): -80ºC; 6 months+
Freeze/Thaw Cycles	1 additional freeze/thaw cycle maximum
Thawing Conditions	Monomer film and Pre-formed Fibrils (PFFs): Room temperature Monomer re-suspension, Monomer in solution, and Oligomers: 4ºC or on ice
Storage (Thawed Protein)	Monomer film: Amyloid beta monomers (catalog# SPR-485) are shipped as a dried peptide film. They must be re-suspended prior to use. Re-suspended product should be used immediately to avoid aggregation. If re-frozen at -80ºC, re-suspended product should be spun down ≥14,000 xg at 4ºC for ≥5 minutes to remove any aggregate formed during freeze/thaw cycle. Monomer in solution and Oligomers: 4^oC or on ice. Use immediately. Pre-formed Fibrils (PFFs): Room temperature. Do not store Pre-formed Fibrils (PFFs) on ice or at 4ºC.
Mixing/Pipetting	Amyloid Beta Pre-formed Fibrils (PFFs), Oligomers, and Monomers that are in solution should be pipetted up and down immediately before use to ensure homogeneity. For Amyloid Beta Monomer preps that are sold as a dried peptide film, once re-suspended into a solution they should be pipetted up and down to ensure homogeneity.
Sonication	For best results, Pre-formed Fibrils (PFFs) should be sonicated immediately prior to use. Mixing/pipetting is also needed before and after sonication to ensure homogeneity.
Safety	Appropriate laboratory attire: Gloves, face mask (VWR, 414004-670), and protective goggles should be used for all work involving fibrils, especially where aerosols may be created (for example during probe sonication). Clean any spills with a solution of 10% bleach or 1M NaOH.