Anti-Alpha Synuclein N-terminal Antibody [11D4]

Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal IgG1

Catalog No. SMC-621

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Species Reactivity Hu, Ms, Rt
Applications WB IHC ICC/IF FCM IP
SKU: SMC-621 Categories: ,

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SMC-621_Alpha-Synuclein-N-terminal_Antibody_11D4_WB_Human_Recombinant-Protein_1.png
Mouse Anti-Alpha Synuclein N-terminal Antibody [11D4] used in Immunohistochemistry (IHC) on HumanBrain (SMC-621)Mouse Anti-Alpha Synuclein N-terminal Antibody [11D4] used in Dot Blot (DB) on HumanRecombinant Protein (SMC-621)Mouse Anti-Alpha Synuclein N-terminal Antibody [11D4] used in Western Blot (WB) on Rat, Mousebrain lysate (SMC-621)
Product Name Alpha Synuclein N-terminal Antibody
Description

Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal IgG1
Species Reactivity Human, Mouse, Rat
Applications WB, IHC
Antibody Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Alpha synuclein aa 1-20
Concentration 1mg/mL
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Dylight 488
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1011 g/mol

Dylight 488 Datasheet

 Dylight 488 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 493 nm

λem = 518 nm

εmax = 7.0×104

Laser = 488 nm

 

APC/Cy7
Overview:

  • High quantum yield
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested

APC-Cy7 Datasheet

 

 ACP-Cy7 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 652 nm

λem = 790 nm

Laser = 594 or 633 nm

 

 

  Dylight 350
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent solubility in water
  • Stringently QC tested
  • Excellent batch-to-batch reproducibility
  • Molecular weight: 874 g/mol

Dylight 350 Datasheet

 Dylight 350 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 353 nm

λem = 432 nm

εmax = 1.5×104

 

 

  Dylight 405
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 793 g/mol

Dylight 405 Datasheet

 Dylight 405 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 400 nm

λem = 420 nm

εmax = 3.0×104

Laser = 405 nm

 

Dylight 594
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1078 g/mol

Dylight 594 Datasheet

 Dylight 594 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 593 nm

λem = 618 nm

εmax = 8.0×104

Laser = 526 nm

 

 Dylight 633
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1066 g/mol

Dylight 633 Datasheet

 Dylight 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 638 nm

λem = 658 nm

εmax = 1.7×105

Laser = 633 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number 11D4
Isotype IgG1
Specificity Detects the N-terminal region of alpha synuclein
Cite This Product Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal (StressMarq Biosciences, Victoria BC, Cat# SMC-621)
Certificate of Analysis A 1:1000 dilution was sufficient for detection of Alpha Synuclein in rodent brain lysates in WB and 1:100 dilution on human brain tissue (Parkinson’s) in IHC.

Biological Description

Alternative Names Alpha Synuclein Antibody, Non-A beta component of AD amyloid Antibody, Non-A4 component of amyloid precursor Antibody, NACP Antibody, SNCA Antibody, PARK1 Antibody, PARK 1 Antibody, alphaSYN Antibody, PARK 4 antibody, PARK4 antibody, Parkinson disease familial 1 antibody, Parkinson disease (autosomal dominant, Lewy body) 4 antibody, SYN antibody
Research Areas Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease, Synuclein, Tangles & Tau, Multiple System Atrophy
Cellular Localization Cytoplasm; membrane-bound to vesicles in neurons
Accession Number P37840
Gene ID 6622
Swiss Prot P37840
Scientific Background Alpha-synuclein undergoes various post-translational modifications, including C-terminal truncations, which can significantly impact its aggregation and pathology in neurodegenerative diseases (1). These truncations are enriched in Lewy bodies and Lewy neurites, accelerating aggregation and enhancing prion-like spreading in Parkinson's disease models. Notably, antibodies targeting phosphorylated alpha-synuclein at serine 129 (PS129) may not detect C-terminal truncated forms, as these modifications can alter the epitope recognized by the antibody. This highlights the importance of selecting appropriate antibodies for detecting specific alpha-synuclein species in research (3).
References 1.,Mahul-Mellier, A.-L., Altay, M. F., Maharjan, N., et al. (2024). Dissecting the differential role of C-terminal truncations in the regulation of aSyn pathology formation and the biogenesis of Lewy bodies. bioRxiv. DOI: 10.1101/2024.11.29.625993
2.,Bell, R., Vendruscolo, M. (2021). Modulation of the interactions between α-synuclein and lipid membranes by post-translational modifications. Frontiers in Neurology, 12, 661117. DOI: 10.3389/fneur.2021.661117
3.,Iyer, A., Roeters, S. J., Kogan, V., Woutersen, S., Claessens, M. M. A. E., & Subramaniam, V. (2017). C-Terminal Truncated α-Synuclein Fibrils Contain Strongly Twisted β-Sheets. Journal of the American Chemical Society, 139(43), 15392-15400. DOI: 10.1021/jacs.7b07403

Product Images

<p>Western blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomers (SPR-321 & SPR-316). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) or Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 610-103-121) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 0.5 second.</p>

Western blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomers (SPR-321 & SPR-316). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) or Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 610-103-121) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 0.5 second.

<p>Immunohistochemistry analysis with Stressmarq’s Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). Tissue: Striatum sections of Parkinson’s Diseased Brain. Species: Human. Fixation: Paraffin embedded, 5um thin. Blocking: 3% milk for 1 hour at RT. Antigen Retrieval: Citrate buffer, pH 6.0, boiled for 20 min. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.</p>

Immunohistochemistry analysis with Stressmarq’s Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). Tissue: Striatum sections of Parkinson’s Diseased Brain. Species: Human. Fixation: Paraffin embedded, 5um thin. Blocking: 3% milk for 1 hour at RT. Antigen Retrieval: Citrate buffer, pH 6.0, boiled for 20 min. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.

<p>Dot blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 and SMC-532 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomer (SPR-321). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621), Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) or Mouse Anti-Mouse Alpha Synuclein Monoclonal (SMC-532) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 710-1331) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 1 second or 5 seconds.</p>

Dot blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 and SMC-532 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomer (SPR-321). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621), Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) or Mouse Anti-Mouse Alpha Synuclein Monoclonal (SMC-532) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 710-1331) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 1 second or 5 seconds.

<p>Western blot analysis with Stressmarq’s SMC-621 showing detection of Alpha Synuclein protein in rat brain and mouse brain lysates. Block: 5% skim milk for 1/2 hour at RT. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (Rockland Cat# 610-103-121) at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 3 seconds (A), cropped and followed by a 1 minute exposure (B) to develop the monomer band in the rat brain lysate.</p>

Western blot analysis with Stressmarq’s SMC-621 showing detection of Alpha Synuclein protein in rat brain and mouse brain lysates. Block: 5% skim milk for 1/2 hour at RT. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (Rockland Cat# 610-103-121) at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 3 seconds (A), cropped and followed by a 1 minute exposure (B) to develop the monomer band in the rat brain lysate.

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