Storage Buffer | PBS pH 7.4, 0.02% Proclin 300, 50% glycerol |
Storage Temperature | -20ºC |
Shipping Temperature | Blue Ice or 4ºC |
Purification | AmMagTM Ultra AT Protein A MagBeads |
Clonality | Recombinant Monoclonal |
Clone Number | CAR1.1 |
Isotype | IgG Kappa |
Specificity | Detects region of v1.3 containing the cytoplasmic C-terminus |
Cite This Product | Kv1.3 Antibody, Clone CAR1.1 (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-630) |
Certificate of Analysis | 1 µg/ml was sufficient for detection of Kv1.3 on Rat Brain Membrane lysate. |
Alternative Names | Kv1.3 Antibody, Potassium voltage-gated channel subfamily A member 3 Antibody, RCK3 Antibody, RGK5 Antibody, Voltage-gated potassium channel subunit Kv1.3 Antibody, Voltage-gated potassium channel subunit Kv3 Antibody, KCNA3 Antibody, KCNA 3 Antibody |
Research Areas | Cancer, Cell Signaling, Ion Channels, Neuroscience, Potassium Channels, Voltage-Gated Potassium Channels |
Cellular Localization | Cell membrane |
Accession Number | NP_062143.3 |
Gene ID | 29731 |
Swiss Prot | P15384 |
Scientific Background | Kv1.3 potassium channels are emerging as critical regulators of neu-roinflammation and neurodegeneration, particularly through their role in microglial activation. In Alzheimer’s disease models, Kv1.3 was shown to be upregulated in activated microglia, and its selective inhibi-tion using HsTX1[R14A] improved cognitive function and reduced pro-inflammatory gene expression in vivo (1). Kv1.3 has also been shown to be upregulated in microglia in response to aggregated alpha synuclein (2). More recent studies also demonstrate that Kv1.3 blockade using venom-derived peptides can reduce neuroinflammatory markers and protect against neuronal damage, further supporting its potential as a therapeutic target in neurodegenerative conditions (3). Our Anti-Rat Kv1.3 Recombinant Monoclonal has been shown to detect the target in over-expression lysates, cell lines, and rodent brains. |
References |
1.,Pan, Y., Sun, J., Deveson, D., Mamo, J. C. L., Takechi, R., Norton, R., Jin, L., & Nicolazzo, J. (2022). A transcriptomic study reveals beneficial effects of HsTX1 [R14A] on the cognition of a mouse model of sporadic Alzheimer’s disease. Alzheimer's & Demen-tia. DOI: 10.1002/alz.068693 2.,Sarkar, S., Nguyen, H. M., Malovic, E., Luo, J., Langley, M., Pal-anisamy, B. N., Singh, N., Manne, S., Neal, M., Gabrielle, M., Abdalla, A., Anantharam, P., Rokad, D., Panicker, N., Singh, V., Ay, M., Charli, A., Harischandra, D., Jin, L.-W., Jin, H., Rangaraju, S., Anantharam, V., Wulff, H., & Kanthasamy, A. G. (2020). Kv1.3 modulates neuroinflammation and neurodegeneration in Parkinson’s disease. Journal of Clinical Investigation, 130(8), 4195–4212. DOI: 10.1172/JCI136174 3.,Beraldo-Neto, E., Ferreira, V. F., Vigerelli, H., Fernandes, K. R., Juliano, M. A., Nencioni, A. L. A., & Pimenta, D. C. (2024). Un-raveling neuroprotection with Kv1.3 potassium channel block-ade by a scorpion venom peptide. Scientific Reports. DOI: 10.1038/s41598-024-79152-1 |
Western blot analysis with Stressmarq’s Rabbit Anti-Kv1.3 Monoclonal Antibody, Clone CAR1.1 (SMC-630) showing detection of Human Kv1.3 protein in a transient over-expression (OE) lysate (OriGene LY4194581). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat Kv1.3 Recombinant Monoclonal [CAR1.1] (SMC-630) at 1:10,000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 15 seconds at RT in the dark. Exposed 0.2 seconds.
Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti-Kv1.3 Monoclonal Antibody, Clone CAR1.1 (SMC-630). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Rat Kv1.3 Recombinant Monoclonal [CAR1.1] (SMC-630) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.
Immunohistochemistry analysis with Stressmarq’s Rabbit Anti-Kv1.3 Monoclonal Antibody, Clone CAR1.1 (SMC-630). Tissue: Brain. Species: Mouse. Fixation: Paraffin embedded, 4um thin. Blocking: 3% milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat Kv1.3 Recombinant Monoclonal [CAR1.1] (SMC-630) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.
Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti-Kv1.3 Monoclonal Antibody, Clone CAR1.1 (SMC-630). Tissue: Neuroblastoma cells (Kelly). Species: Human. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Rat Kv1.3 Recombinant Monoclonal [CAR1.1] (SMC-630) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.
Western blot analysis with Stressmarq’s Rabbit Anti-Kv1.3 Monoclonal Antibody, Clone CAR1.1 (SMC-630) showing detection of Rat Kv1.3 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Rat Kv1.3 Recombinant Monoclonal [CAR1.1] (SMC-630) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 20 seconds.
Reviews
There are no reviews yet.