RECOMBINANT
Anti-Slo1 Antibody [S22]

Rabbit Anti-Mouse Slo1 Recombinant Monoclonal IgG Kappa

Catalog No. SMC-625

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Species Reactivity Hu, Ms, Rt
Applications WB IHC ICC/IF FCM IP
SKU: SMC-625 Categories: ,

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SMC-625_Slo1_Antibody_S22_WB_Human-OE-lysate_1.png
Rabbit Anti-Slo1 Antibody [S22] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Human Neuroblastoma Cells (SMC-625)Rabbit Anti-Slo1 Antibody [S22] used in Immunohistochemistry (IHC) on Mouse Brain (SMC-625)Rabbit Anti-Slo1 Antibody [S22] used in Immunocytochemistry/Immunofluorescence (ICC/IF) on Rat Pheochromocytoma Cells (SMC-625)Rabbit Anti-Slo1 Antibody [S22] used in Western Blot (WB) on Rat Brain membrane lysate (SMC-625)
Product Name Slo1 Antibody
Description

Rabbit Anti-Mouse Slo1 Recombinant Monoclonal IgG Kappa
Species Reactivity Human, Mouse, Rat
Applications WB, IHC, ICC/IF
Antibody Dilution WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.
Host Species Rabbit
Immunogen Species Mouse
Immunogen Fusion protein, AA #690-1196 (cytoplasmic C-terminus) of mouse Slo1. Epitope mapped to within AA #690-715 (PKLMRHDPLLIPGNDQIDNMDSNVKK).
Concentration 1mg/mL
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Dylight 488
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1011 g/mol

Dylight 488 Datasheet

 Dylight 488 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 493 nm

λem = 518 nm

εmax = 7.0×104

Laser = 488 nm

 

APC/Cy7
Overview:

  • High quantum yield
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested

APC-Cy7 Datasheet

 

 ACP-Cy7 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 652 nm

λem = 790 nm

Laser = 594 or 633 nm

 

 

  Dylight 350
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent solubility in water
  • Stringently QC tested
  • Excellent batch-to-batch reproducibility
  • Molecular weight: 874 g/mol

Dylight 350 Datasheet

 Dylight 350 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 353 nm

λem = 432 nm

εmax = 1.5×104

 

 

  Dylight 405
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 793 g/mol

Dylight 405 Datasheet

 Dylight 405 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 400 nm

λem = 420 nm

εmax = 3.0×104

Laser = 405 nm

 

Dylight 594
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1078 g/mol

Dylight 594 Datasheet

 Dylight 594 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 593 nm

λem = 618 nm

εmax = 8.0×104

Laser = 526 nm

 

 Dylight 633
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1066 g/mol

Dylight 633 Datasheet

 Dylight 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 638 nm

λem = 658 nm

εmax = 1.7×105

Laser = 633 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications. 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications. 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA*

*The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA*
    • *The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

 

  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH 7.4, 0.02% Proclin 300, 50% glycerol
Storage Temperature -20ºC, Conjugated antibodies should be stored according to the product label
Shipping Temperature Blue Ice or 4ºC
Purification AmMagTM Ultra AT Protein A MagBeads
Clonality Recombinant Monoclonal
Clone Number S22
Isotype IgG Kappa
Specificity Detects C-terminus of Slo1, epitope mapped to within AA #690-715 (PKLMRHDPLLIPGNDQIDNMDSNVKK).
Cite This Product Rabbit Anti-Mouse Slo1 Recombinant Monoclonal (StressMarq Biosciences, Victoria BC, Cat# SMC-625)
Certificate of Analysis 1 µg/ml was sufficient for detection of Slo1 on Rat Brain Membrane lysate.

Biological Description

Alternative Names Slo1, BKAlpha, maxi-K+ channel, Calcium-activated potassium channel subunit alpha-1, Calcium-activated potassium channel, Subfamily M subunit alpha-1, KCa1.1, K(VCA)alpha, BK channel, BKCA alpha, Maxi K channel, Slo-alpha, Slowpoke homolog, Kcnma1, Kcnma, Maxi Potassium channel alpha, KCMA1, Slo, BKCa, mSlo, MaxiK, mSlo1, 5730414M22Rik, BK, BKCA channel, K VCA, BKCA alpha subunit, BKTM, Drosophila slowpoke like, SAKCA
Research Areas Calcium-Activated Potassium Channels, Cancer, Cardiovascular System, Cell Signaling, Ion Channels, Neuroscience, Potassium Channels
Cellular Localization Cell Membrane, Nucleus
Accession Number AAA39746.1
Gene ID 16531
Swiss Prot Q08460
Scientific Background Slo1, also known as the BK (Big Potassium) channel or BKα, is a voltage- and calcium-activated potassium channel highly expressed in neurons and smooth muscle. It plays a critical role in regulating membrane excitability by facilitating the repolarization phase of action potentials and modulating neurotransmitter release.

Slo1 channel activity is finely tuned by intracellular Ca²⁺ levels and membrane potential, and is further regulated by auxiliary KCNMB subunits. This dynamic regulation enables Slo1 to influence synaptic transmission, motor coordination, and responses to ethanol. Notably, Slo1 dysfunction has been implicated in a range of neurological and neuromuscular disorders, including epilepsy, ataxia, hypertension, and muscular dystrophy.

Recent studies suggest that Slo1 may also contribute to neurodegenerative disease mechanisms by altering neuronal firing patterns and calcium homeostasis. Its interaction with dystrophin-related pathways further underscores its relevance in neuromuscular integrity.

Given its broad physiological roles and disease associations, Slo1 is emerging as a promising target for therapeutic intervention and biomarker development in neurodegenerative research.
References 1.,Yang, C.T., Zeng X.H., Xia X.M., and Lingle C.J. (2009). Interactions between β Subunits of the KCNMB Family and Slo3: β4 Selectively Modulates Slo3 Expression and FunctionPloS One. 4(7): e6135. DOI: 10.1371/journal.pone.0006135
2.,Latorre, R., & Brauchi, S. (2006). Large conductance Ca2+-activated K+ (BK) channel: Activation by Ca2+ and voltage. Biological Research, 39(3), 385-401. DOI: 10.4067/s0716-97602006000300003
3.,Petkov G. V. (2014). Central role of the BK channel in urinary bladder smooth muscle physiology and pathophysiology. American journal of physiology. Regulatory, integrative and comparative physiology, 307(6), R571–R584. DOI: 10.1152/ajpregu.00142.2014
4.,Pathak, D., Guan, D., & Foehring, R. C. (2016). Roles of specific Kv channel types in repolarization of the action potential in genetically identified subclasses of pyramidal neurons in mouse neocortex. Journal of neurophysiology, 115(5), 2317–2329. DOI: 10.1152/jn.01028.2015
5.,Kim, H., Pierce-Shimomura, J. T., Oh, H. J., Johnson, B. E., Goodman, M. B., & McIntire, S. L. (2009). The dystrophin complex controls bk channel localization and muscle activity in Caenorhabditis elegans. PLoS genetics, 5(12), e1000780. DOI: 10.1371/journal.pgen.1000780

Product Images

<p>Western blot analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625) showing detection of Human Slo1 protein in a transient over-expression (OE) lysate (OriGene LY400815). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:1000 for 2 hours at RT. Secondary Antibody: , at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 30 sec at RT in the dark. Exposed 15 seconds. NOTE: The ORF clone contains a C-term DDK tag; results were confirmed using Anti-DDK antibody (OriGene TA592569), showing laddering above target is related to homooligomerization of the Slo1 protein (data not shown).</p>

Western blot analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625) showing detection of Human Slo1 protein in a transient over-expression (OE) lysate (OriGene LY400815). Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:1000 for 2 hours at RT. Secondary Antibody: , at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 30 sec at RT in the dark. Exposed 15 seconds. NOTE: The ORF clone contains a C-term DDK tag; results were confirmed using Anti-DDK antibody (OriGene TA592569), showing laddering above target is related to homooligomerization of the Slo1 protein (data not shown).

<p>Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Neuroblastoma cells (Kelly). Species: Human. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.</p>

Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Neuroblastoma cells (Kelly). Species: Human. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.

<p>Immunohistochemistry analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Brain. Species: Mouse. Fixation: Paraffin embedded, 4um thin. Blocking: 3% milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP(H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.</p>

Immunohistochemistry analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Brain. Species: Mouse. Fixation: Paraffin embedded, 4um thin. Blocking: 3% milk for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-rabbit IgG:HRP(H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.

<p>Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.</p>

Immunocytochemistry/Immunofluorescence analysis with Stressmarq’s Rabbit Anti- Slo1 Monoclonal Antibody, Clone S22 (SMC-625). Tissue: Pheochromocytoma cells (PC-12). Species: Rat. Fixation: 4% PFA for 10 min at RT. Permeabilization: 0.15% TritonX for 15 min at RT. Blocking: 10% goat serum for 40 min at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-Rabbit IgG-AlexaFluor488 (green) at 1:1000 for 1 hour at RT in the dark. Counterstain: DAPI (blue) nuclear stain at 1:1000 for 5 min at RT in the dark.

<p>Western blot analysis with Stressmarq’s Rabbit Anti-Slo1 Monoclonal Antibody, Clone S22 (SMC-625) showing detection of Rat Slo1 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 6.5 seconds.</p>

Western blot analysis with Stressmarq’s Rabbit Anti-Slo1 Monoclonal Antibody, Clone S22 (SMC-625) showing detection of Rat Slo1 protein in a Rat brain membrane lysate. Block: 2% skim milk + 2% BSA for 1 hour at RT. Primary Antibody: Rabbit Anti-Mouse Slo1 Recombinant Monoclonal [S22] (SMC-625) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat anti-rabbit IgG-HRP (H&L) at 1:5000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 6.5 seconds.

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