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| Product Name | Alpha Synuclein Oligomer ELISA Kit |
| Description |
Colorimetric detection of oligomeric alpha synuclein |
| Species Reactivity | Human, Mouse |
| Platform | Microplate |
| Sample Types | Brain homogenate |
| Detection Method | Colorimetric Assay |
| Assay Type | Sandwich ELISA |
| Utility | ELISA kit used to detect/quantitate the presence of alpha synuclein oligomers |
| Sensitivity | 10 pg/mL |
| Assay Range | 15.6 – 1,000 pg/mL |
| Incubation Time | 3 hours |
| Number of Samples | 40 samples in duplicate |
| Other Resources | Kit Booklet |
| Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
Properties
| Storage Temperature | 4°C/-20°C |
| Shipping Temperature | Blue Ice |
| Product Type | ELISA Kits |
| Assay Overview | 1.,Prepare Standard and samples in Standard and Sample Diluent. 2.,Add 100 µL of Standard or sample to appropriate wells. 3.,Cover plate with Plate Sealer and incubate at Room Tempterature for 1 hour with shaking at 600rpm. 4.,Wash plate four times with 1X Wash Buffer. 5.,Add 100 µL of Biotinylated Antibody Working Solution to each well. 6.,Cover plate with Plate Sealer and incubate at Room Temperature for 1 hour with shaking at 600rpm 7.,Wash plate four times with 1X Wash Buffer. 8.,Add 100 µL of Streptavidin HRP Working Solution to each well. 9.,Cover plate with Plate Sealer and incubate at Room Temperature for 30 minutes with shaking at 600rpm. 10.,Wash plate four times with 1X Wash Buffer. 11.,Add 100 µL of TMB Substrate to each well. |
| Cite This Product | Alpha Synuclein Oligomer ELISA Kit (StressMarq Biosciences | Victoria, BC CANADA | Catalog# SKT-143-96) |
Biological Description
| Alternative Names | α syn oligomers, aSyn oligomers, prefibrillar α syn aggregates, alpha synuclein, α syn, aSyn, SNCA, Non A4 / Non Aβ Component of AD Amyloid, NACP, PARK1, PARK4 |
| Research Areas | Acetylation, Neurodegeneration, Neuroscience, Parkinson's Disease, Synuclein, Multiple System Atrophy |
| Scientific Background | Alpha synuclein oligomers represent a soluble, neurotoxic form of the presynaptic protein and are increasingly recognized as early drivers of pathology in neurodegenerative disease. These oligomeric species disrupt synaptic vesicle trafficking and neurotransmission, impairing presynaptic function well before the formation of mature fibrillar aggregates. Localized effects extend to mitochondria, where oligomers induce oxidative stress, lysosomal dysfunction, and endoplasmic reticulum stress, contributing to neuronal vulnerability in Parkinson’s disease (PD) and related synucleinopathies. Their capacity to propagate in a prion like manner and initiate early synaptic and inflammatory disturbances underscores their importance in disease progression and positions them as high value targets for biomarker development and therapeutic intervention. StressMarq’s Alpha Synuclein Oligomer ELISA has demonstrated higher affinity to oligomers than to PFFs or monomers. |
| References |
1.,Ghiglieri, V., Calabrese, V., & Calabresi, P. (2018). Alpha-Synuclein: From Early Synaptic Dysfunction to Neurodegeneration. Frontiers in neurology, 9, 295. https://doi.org/10.3389/fneur.2018.00295 2.,Pitton Rissardo, J., McGarry, A., Shi, Y., Fornari Caprara, A. L., & Kannarkat, G. T. (2025). Alpha-Synuclein Neurobiology in Parkinson’s Disease: A Comprehensive Review of Its Role, Mechanisms, and Therapeutic Perspectives. Brain Sciences, 15(12), 1260. https://doi.org/10.3390/brainsci15121260 3.,Carvalho, R. (2025). Alpha Synuclein: A key player in neurodegenerative disorders. Journal of Neuroinfectious Diseases, 16(1). https://www.omicsonline.org/open-access-pdfs/alphasynuclein-a-key-player-in-neurodegenerative-disorders.pdf |
Product Images
Typical Standard Curve for the Alpha Synuclein Oligomer ELISA Kit (SKT-143). Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 15.6 – 1,000 pg/ml.
The graph demonstrates robust detection of Human Recombinant Alpha Synuclein Oligomers (Cat # SPR-484) and limited cross-reactivity with Human Recombinant Alpha Synuclein Pre-Formed Fibrils and Monomers (Cat #s SPR-322 & SPR-321).
Representative quantification of mouse brain homogenate samples of unknown concentrations of Alpha Synuclein Oligomers against a standard curve of Human Recombinant Alpha Synuclein Oligomers when using StressMarq’s Alpha Synuclein Oligomer ELISA kit (Cat # SKT-143). A: Non-detergent protein extraction from brain homogenates of hemizygous M83 mice injected in the striatum with either Human Recombinant Alpha Synuclein Pre-Formed Fibrils (PFF; n = 3) or with buffer (PBS; n = 3). B: Non-detergent protein extraction from brain homogenates of either homozygous M83 mice (M83 Transgenic; n = 2) or wildtype mice (Wildtype; n = 2). C-D: Detergent-based protein extraction (25 ug/mL) from brain homogenates of hemizygous M83 mice injected in the striatum with either PFF or PBS. Data from males (C: PFF n = 6; PBS n = 6) and females (D: PFF n = 6; PBS n = 5) are presented separately. Experimental data courtesy of Rodrigo Sandoval Contreras and Dareen Abdel-Rahman from the Prado Lab at University of Western Ontario, contributed as part of a collaborative research project.
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