StressXpress®
HSP65 ELISA Kit

Colorimetric detection of HSP65

Catalog No. SKT-113

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Species Reactivity Ba,
Sample Types Cell lysates, Serum, Tissue
SKU: SKT-113 Categories: ,

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Product Name HSP65 ELISA Kit
Description

Colorimetric detection of HSP65

Species Reactivity Mycobacterium Bovis, Mycobacterium Tuberculosum
Platform Microplate
Sample Types Cell lysates, Serum, Tissue
Detection Method Colorimetric Assay
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Utility ELISA kit used to quantitate M. bovis/M. tuberculosum HSP65 concentration in human samples
Sensitivity 0.06 ng/ml
Assay Range 0.344 - 22 ng/ml
Incubation Time 30 minutes
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet , MSDS
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Assay Overview 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Kit Overview
Component No.ItemQuantity / Size
SKC-113A Anti-Hsp65 Immunoassay Plate 1 Plate
SKC-113B 5X Hsp65 Extraction Reagent 1 vial/10 ml
SKC-113C Recombinant Hsp65 Standard 2 vials
SKC-113D Standard and Sample Diluent 1 vial/ 50 ml
SKC-113E 10X Wash Buffer Concentrate 1 vial/100 ml
SKC-113F Anti-Hsp65 Biotinylated Antibody Concentrate 1 vial/150 µl
SKC-113G Anti-Hsp65 Biotinylated Antibody Diluent 1 vial/ 13 ml
SKC-113H Streptavidin: HRP Concentrate 1 vial/150 µl
SKC-113I Streptavidin: HRP Diluent 1 vial/ 13 ml
SKC-113J TMB Substrate 1 vial/ 13 ml
SKC-113K Stop Solution 1 vial/ 13 ml
Cite This Product HSP65 ELISA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-113)

Biological Description

Alternative Names 60kDa chaperonin 2 ELISA Kit, Cell wall protein A ELISA Kit, groEL Protein 2 ELISA Kit, GroEL2 ELISA Kit, M. Tuberculosis cell wall protein A ELISA Kit, M. Tuberculosis HSP65 ELISA Kit
Research Areas Cancer, Heat Shock
Scientific Background HSP65 is a member of the HSP60 family of heat shock proteins (2, 3). HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner (1). Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (2,4). Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (5, 6).
References 1. Koll H., et al. (1992) Cell. 68: 1163-1175.
2. Thole J.E.R., et al. (1985) Infect. Immuno. 50: 800-806.
3. Thole J.E.R., et al., (1987) Infect. Immuno. 55: 1466-1475.
4. Shinnick T.M. Sweetser D., Thole J., van Embden J. and Young R.A. (1987) Infect. Immuno. 55: 1932-1935.
5. Van Eden W., et al. (1988) Nature 331: 171-178.
6. Cobelens P.M., et al. (2002) Rheumatology 41: 775-779.

Product Images

<p>Typical Standard Curve for the HSP65 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-113. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.344 – 22 ng/mL.</p>

Typical Standard Curve for the HSP65 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-113. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.344 – 22 ng/mL.

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