Product Name | HSP65 ELISA Kit |
Description |
Colorimetric detection of HSP65 |
Species Reactivity | Mycobacterium Bovis, Mycobacterium Tuberculosum |
Platform | Microplate |
Sample Types | Cell lysates, Serum, Tissue |
Detection Method | Colorimetric Assay |
Assay Type | Sandwich ELISA (Enzyme-linked Immunosorbent Assay) |
Utility | ELISA kit used to quantitate M. bovis/M. tuberculosum HSP65 concentration in human samples |
Sensitivity | 0.06 ng/ml |
Assay Range | 0.344 - 22 ng/ml |
Incubation Time | 30 minutes |
Number of Samples | 40 samples in duplicate |
Other Resources | Kit Booklet , MSDS |
Field of Use | Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only. |
Storage Temperature | 4ºC | ||||||||||||||||||||||||||||||||||||
Shipping Temperature | Blue Ice | ||||||||||||||||||||||||||||||||||||
Product Type | ELISA Kits | ||||||||||||||||||||||||||||||||||||
Assay Overview | 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at room temperature (20-25°C) for 1 hour. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at room temperature for 1 hour. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at room temperature for 30 minutes. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm. | ||||||||||||||||||||||||||||||||||||
Kit Overview |
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Cite This Product | HSP65 ELISA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-113) |
Alternative Names | 60kDa chaperonin 2 ELISA Kit, Cell wall protein A ELISA Kit, groEL Protein 2 ELISA Kit, GroEL2 ELISA Kit, M. Tuberculosis cell wall protein A ELISA Kit, M. Tuberculosis HSP65 ELISA Kit |
Research Areas | Cancer, Heat Shock |
Scientific Background | HSP65 is a member of the HSP60 family of heat shock proteins (2, 3). HSP60s are mitochondrial chaperonins that are typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. HSP60s are a ubiquitous class of HSPs that specifically promote the folding and assembly of cellular polypeptides in an ATP-dependent manner (1). Specifically, sequence comparison of HSP65 from different mycobacterium strains showed that the protein sequence of M. bovis BCG is identical to that of M. tuberculosis, and very similar to that of M. leprae, the pathogens that cause tuberculosis and tuberculoid leprosy, respectively (2,4). Mycobacterium bovis BCG HSP65 was identified as the immunodominant antigen during mycobacterial diseases and vaccination. It is also believed to be the antigen that induces autoimmune disease, such as adjuvant arthritis in rats (5, 6). |
References |
1. Koll H., et al. (1992) Cell. 68: 1163-1175. 2. Thole J.E.R., et al. (1985) Infect. Immuno. 50: 800-806. 3. Thole J.E.R., et al., (1987) Infect. Immuno. 55: 1466-1475. 4. Shinnick T.M. Sweetser D., Thole J., van Embden J. and Young R.A. (1987) Infect. Immuno. 55: 1932-1935. 5. Van Eden W., et al. (1988) Nature 331: 171-178. 6. Cobelens P.M., et al. (2002) Rheumatology 41: 775-779. |
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