StressXpress®
HSP70 ELISA Kit (High-Sensitivity)

High-Sensitivity Colorimetric detection of HSP70

Catalog No. SKT-108

5.00 out of 5 based on 1 customer rating
Species Reactivity Hu, Mk, Dg, Rt, Ms
Sample Types Cell lysates, Plasma, Serum, Tissue
SKU: SKT-108 Categories: ,

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SKT-108_Hsp70_High-Sensitivity_ELISA_Kit_Standard_curve.png
Product Name HSP70 ELISA Kit (High-Sensitivity)
Description

High-Sensitivity Colorimetric detection of HSP70

Species Reactivity Dog, Human, Monkey, Mouse, Rat
Platform Microplate
Sample Types Cell lysates, Plasma, Serum, Tissue
Detection Method Colorimetric Assay
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Utility ELISA kit used to quantitate HSP70 concentration in samples.
Sensitivity 0.038 ng/ml
Assay Range 0.42 - 27 ng/ml
Incubation Time 30 minutes
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet Lot No. VH853927 , Kit Booklet Lot No. VH477277 , Kit Booklet Lot No. VH777390 , Kit Booklet Lot No. VH288828 , MSDS
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Assay Overview 1. Prepare Standard and samples in Standard and Sample Diluent. 2. Add 100 µL of Standard or sample to appropriate wells. 3. Cover plate with Plate Sealer and incubate at 37°C for 1 hour with shaking at 600rpm. 4. Wash plate four times with 1X Wash Buffer. 5. Add 100 µL of Biotinylated Antibody Working Solution to each well. 6. Cover plate with Plate Sealer and incubate at at 37°C for 1 hour with shaking at 600rpm. 7. Wash plate four times with 1X Wash Buffer. 8. Add 100 µL of Streptavidin-HRP Working Solution to each well. 9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes with shaking at 600rpm. 10. Wash plate four times with 1X Wash Buffer. 11. Add 100 µL of TMB Substrate to each well. 12. Develop the plate in the dark at room temperature for 30 minutes. 13. Stop reaction by adding 100 µL of Stop Solution to each well. 14. Measure absorbance on a plate reader at 450 nm.
Kit Overview
Component No.ItemQuantity / Size
SKC-108A Anti-Hsp70 Immunoassay Plate 1 Plate
SKC-108B Recombinant Hsp70 Standard 2 vials
SKC-108C Standard and Sample Diluent 1 vial/ 50 ml
SKC-108D 10X Wash Buffer Concentrate 1 vial/100 ml
SKC-108E Anti-Hsp70 Biotinylated Antibody Concentrate 1 vial/150 µl
SKC-108F Anti-Hsp70 Biotinylated Antibody Diluent 1 vial/ 13 ml
SKC-108G Streptavidin: HRP Concentrate 1 vial/50 µl
SKC-108H Streptavidin: HRP Diluent 1 vial/ 13 ml
SKC-108I TMB Substrate 1 vial/ 13 ml
SKC-108J Stop Solution 1 vial/ 13 ml
Cite This Product HSP70 ELISA Kit (High-Sensitivity) (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-108)

Biological Description

Alternative Names HSP70 1, HSP70 2, HSP70.1, HSP72, HSPA1, HSPA1A, HSPA1B
Research Areas Cancer, Cell Signaling, Chaperone Proteins, Heat Shock, Protein Trafficking, Tumor Biomarkers
Scientific Background Heat Shock Protein 70 (HSP70) is a highly conserved molecular chaperone encoded by a multigene family and expressed across all major cellular compartments, including the cytosol, mitochondria, and endoplasmic reticulum. HSP70 binds to nascent and misfolded proteins, preventing aggregation and facilitating proper folding through ATP-dependent conformational changes.

In neuroscience, HSP70 plays a critical role in maintaining proteostasis under stress conditions. Its ability to stabilize unfolded proteins is especially relevant in neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and ALS, where protein misfolding and aggregation are central to pathogenesis. HSP70’s dynamic binding and release cycles are essential for neuronal survival, synaptic function, and cellular resilience. By modulating HSP70 activity, researchers aim to enhance the brain’s natural defense mechanisms against proteotoxic stress, making it a promising therapeutic target in neurodegeneration.
References 1. Zho J. (1998) Cell. 94: 471-480.
2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993) J. Mol. Evol. 38 (1) 1-17.
3. Rothman J. (1989) Cell 59: 591 -601.
4. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887.
5. Bork P., Sander C. & Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294.
6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
7. Smith D.F., et al. (1993) Mol. Cell. Biol. 13(2): 869-876.
8. Prapapanich V., et al. (1996) Mol. Cell. Biol. 16(11): 6200-6207.
9. Fernandez-Funez et al. (2000) Nature. 408(6808): 101-106.

Product Images

<p>Typical Standard Curve for the HSP70 ELISA Kit (High-Sensitivity) (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-108. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.55 – 35 ng/mL.</p>

Typical Standard Curve for the HSP70 ELISA Kit (High-Sensitivity) (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-108. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.55 – 35 ng/mL.

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