Protocols | TEM

TEM (Transmission Electron Microscopy)

This method describes general TEM analysis of protein samples with negative staining.

Procedure
  1. Thaw the sample.
  2. Apply 10 ul of the sample onto a carbon filmed grid with the excess liquid being removed by blotting a piece of filter paper after 1 minute.
  3. Apply 10 ul of dH2O onto the grid and remove the excess liquid with filter paper.
  4. Apply 10 ul of 2% Uranyl Acetate onto the grid and remove the excess liquid with filter paper after 30 seconds.
  5. Apply another 10 ul of 2% Uranyl Acetate onto the grid and remove the excess liquid with filter paper after 1 minute.
  6. Allow the grid air dry for a few minutes and store it in a grid box.
  7. The specimen is examined and photographed on Thermo Scientific Talos L120C transmission electron microscope at an accelerating voltage of 120Kv.
Supplies
  • Uranyl Acetate: EMS, Cat# 22400
  • Carbon 400 mesh grids: EMS, Cat# CF400-Cu-TH