Storage Buffer | PBS pH7.4, 50% glycerol, 0.1% sodium azide *Storage buffer may change when conjugated |
Storage Temperature | -20ºC, Conjugated antibodies should be stored according to the product label |
Shipping Temperature | Blue Ice or 4ºC |
Purification | Protein G Purified |
Clonality | Monoclonal |
Clone Number | N393/2 (Formerly sold as S393-2) |
Isotype | IgG1 |
Specificity | Detects ~>200kDa. Does not cross react with other Beta-spectrins |
Cite This Product | StressMarq Biosciences Cat# SMC-484, RRID: AB_2702423 |
Certificate of Analysis | A 1:100 dilution of SMC-484 was sufficient for detection of Beta 4 Spectrin in 20 µg of mouse brain lysate by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. |
Alternative Names | BetaIV-spectrin sigma1 Antibody, Non-erythrocytic spectrin beta chain 4 Antibody, Non-erythroidspectrin beta chain 3 Antibody, SPTBN4 Antibody, SPTBN3 Antibody, Spnb4 Antibody, KIAA1642 Antibody, EMBL BAB83243.1 Antibody, Ensembl ENSRNOP00000028393 Antibody |
Research Areas | Cell Signaling, Neuroscience, Scaffold Proteins |
Cellular Localization | Cell Cortex, Cytoplasm, Cytoskeleton |
Accession Number | NP_066022.2 |
Gene ID | 57731 |
Swiss Prot | Q9H254 |
Scientific Background | Spectrin is the major constituent of the cytoskeletal network underlying the erthrocyte plasma membrane and determines cell shape, arrangement of transmembrane proteins and organization of organelles. It is expressed at very low levels in many tissues, with the strongest expression in the cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder and heart (1). Spectrin beta 4 is a non-erythrocyte member, and is expressed in the brain and pancreatic islets and localisted to the nuclear matrix, cytoplasmic vesicles and PML nuclear bodies. Beta4 spectrins are also essential for membrane stability and the molecular organization of the nodes of Ranvier (2). |
References |
1. Genes and mapped phenotypes. (n.d.). Retrieved March 12, 2015, from http://www.ncbi.nlm.nih.gov/gene/57731 2. Yang Y., Lacas-Gervais S., Morest D.K., Solimena M., and Rasband M.N. (2004) J Neurosci. 24(33): 7230-7240. |
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone N393/2 (SMC-484). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (SMC-484) at 1:100 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) beta 4 Spectrin Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-beta 4 Spectrin Monoclonal Antibody, Clone N393/2 (SMC-484). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-beta 4 Spectrin Monoclonal Antibody (SMC-484) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000; 1:5000 for 60 min RT, 5 min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) beta 4 Spectrin Antibody. (D) Composite.
Western Blot analysis of Monkey COS-Beta-4-Spectrin-His showing detection of ~ 200 kDa Beta-4-Spectrin protein using Mouse Anti-Beta-4-Spectrin Monoclonal Antibody, Clone N393/2 (SMC-484). Lane 1: MW Ladder. Lane 2: COS-Beta-4-Spectrin-His. Load: 15 µg. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Beta-4-Spectrin Monoclonal Antibody (SMC-484) at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~ 200 kDa.
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