Anti-Alpha Synuclein N-terminal Antibody [11D4]

Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal IgG1

Catalog No. SMC-621

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Species Reactivity Hu, Ms, Rt
Applications WB IHC ICC/IF FCM IP
SKU: SMC-621 Categories: ,

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SMC-621_Alpha-Synuclein-N-terminal_Antibody_11D4_WB_Human_Recombinant-Protein_1.png
Mouse Anti-Alpha Synuclein N-terminal Antibody [11D4] used in Immunohistochemistry (IHC) on HumanBrain (SMC-621)Mouse Anti-Alpha Synuclein N-terminal Antibody [11D4] used in Dot Blot (DB) on HumanRecombinant Protein (SMC-621)Mouse Anti-Alpha Synuclein N-terminal Antibody [11D4] used in Western Blot (WB) on Rat, Mousebrain lysate (SMC-621)
Product Name Alpha Synuclein N-terminal Antibody
Description

Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal IgG1
Species Reactivity Human, Mouse, Rat
Applications WB, IHC
Antibody Dilution WB (1:1000), IHC (1:100); optimal dilutions for assays should be determined by the user.; optimal dilutions for assays should be determined by the user.
Host Species Mouse
Immunogen Species Human
Immunogen Alpha synuclein aa 1-20
Concentration 1mg/mL
Conjugates APC, ATTO 390, ATTO 488, ATTO 594, Biotin, FITC, HRP, PerCP, RPE, Unconjugated
Dylight 488
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1011 g/mol

Dylight 488 Datasheet

 Dylight 488 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 493 nm

λem = 518 nm

εmax = 7.0×104

Laser = 488 nm

 

APC/Cy7
Overview:

  • High quantum yield
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested

APC-Cy7 Datasheet

 

 ACP-Cy7 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 652 nm

λem = 790 nm

Laser = 594 or 633 nm

 

 

  Dylight 350
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent solubility in water
  • Stringently QC tested
  • Excellent batch-to-batch reproducibility
  • Molecular weight: 874 g/mol

Dylight 350 Datasheet

 Dylight 350 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 353 nm

λem = 432 nm

εmax = 1.5×104

 

 

  Dylight 405
Overview:

  • High fluorescence intensity
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 793 g/mol

Dylight 405 Datasheet

 Dylight 405 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 400 nm

λem = 420 nm

εmax = 3.0×104

Laser = 405 nm

 

Dylight 594
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1078 g/mol

Dylight 594 Datasheet

 Dylight 594 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 593 nm

λem = 618 nm

εmax = 8.0×104

Laser = 526 nm

 

 Dylight 633
Overview:

  • High fluorescence yield
  • High photostability
  • Less pH-sensitive
  • Excellent batch-to-batch reproducibility
  • Stringently QC tested
  • Molecular weight: 1066 g/mol

Dylight 633 Datasheet

 Dylight 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 638 nm

λem = 658 nm

εmax = 1.7×105

Laser = 633 nm

 

 PerCP 
Overview:

  • Peridinin-Chlorophyll-Protein Complex
  • Small phycobiliprotein
  • Isolated from red algae
  • Large stokes shift (195 nm)
  • Molecular Weight: 35 kDa
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

PerCP Datasheet

  PerCP Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 482 nm

λem = 677 nm

εmax = 1.96 x 106

Laser = 488 nm

 

 PE/ATTO 594
PE/ATTO 594 is a tandem conjugate, where PE is excited at 535 nm and transfers energy to ATTO 594 via FRET (fluorescence resonance energy transfer), which emits at 627 nm.
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation

PE/ATTO 594 Datasheet

 PE-ATTO 594 Fluorophore Conjugate Excitation and Emission Spectra Optical Properties:

λex = 535 nm

λem = 627 nm

Laser = 488 to 561 nm

 

  FITC (Fluorescein)
Overview:

  • Excellent fluorescence quantum yield
  • High rate of photobleaching
  • Good solubility in water
  • Broad emission spectrum
  • pH dependent spectra
  • Molecular formula: C20H12O5
  • Molar mass: 332.3 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

FITC-Fluorescent-conjugate

 FITC Fluorescein Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 494 nm

λem = 520 nm

εmax = 7.3×104

Φf = 0.92

τfl = 5.0 ns

Brightness = 67.2

Laser = 488 nm

Filter set = FITC

 

 ATTO 700
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 575 g/mol

ATTO 700 Datasheet

  ATTO 700 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 700 nm

λem = 719 nm

εmax = 1.25×105

Φf = 0.25

τfl = 1.6 ns

Brightness = 31.3

Laser = 676 nm

Filter set = Cy®5.5

 

 ATTO 680
Overview:

  • High fluorescence yield
  • Excellent thermal and photostability
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 631 g/mol

ATTO 680 Datasheet

  ATTO 680 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 680 nm

λem = 700 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.7 ns

Brightness = 37.5

Laser = 633 – 676 nm

Filter set = Cy®5.5

 

 ATTO 655
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Excellent ozone resistance
  • Quenched by electron donors
  • Very hydrophilic
  • Good solubility in polar solvents
  • Zwitterionic dye
  • Molar Mass: 634 g/mol

ATTO 655 Datasheet

 ATTO 655 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 663 nm

λem = 684 nm

εmax = 1.25×105

Φf = 0.30

τfl = 1.8 ns

Brightness = 37.5

Laser = 633 – 647 nm

Filter set = Cy®5

 

 ATTO 633
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Stable at pH 4 – 11
  • Cationic dye, perchlorate salt
  • Molar Mass: 652.2 g/mol

ATTO 633 Datasheet

 ATTO 633 Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 629 nm

λem = 657 nm

εmax = 1.3×105

Φf = 0.64

τfl = 3.2 ns

Brightness = 83.2

Laser = 633 nm

Filter set = Cy®5

 

 ATTO 594
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 1137 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

ATTO 594 Datasheet

  ATTO 594 Fluorophore Excitation and Emission Spectrum Optical Properties:

λex = 601 nm

λem = 627 nm

εmax = 1.2×105

Φf = 0.85

τfl = 3.5 ns

Brightness = 102

Laser = 594 nm

Filter set = Texas Red®

 

 ATTO 565
Overview:

  • High fluorescence yield
  • High thermal and photostability
  • Good solubility in polar solvents
  • Excellent solubility in water
  • Very little aggregation
  • Rhodamine dye derivative
  • Molar Mass: 611 g/mol

ATTO 565 Datasheet

  ATTO 565 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 563 nm

λem = 592 nm

εmax = 1.2×105

Φf = 0.9

τfl = 3.4 n

Brightness = 10

Laser = 532 nm

Filter set = TRITC

 

  ATTO 488
Overview:

  • High fluorescence yield
  • High photostability
  • Very hydrophilic
  • Excellent solubility in water
  • Very little aggregation
  • New dye with net charge of -1
  • Molar Mass: 804 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications. 

ATTO 488 Datasheet

   ATTO 488 Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 501 nm

λem = 523 nm

εmax = 9.0×104

Φf = 0.80

τfl = 4.1 ns

Brightness = 72

Laser = 488 nm

Filter set = FITC

 

  ATTO 390
Overview:

  • High fluorescence yield
  • Large Stokes-shift (89 nm)
  • Good photostability
  • Moderately hydrophilic
  • Good solubility in polar solvents
  • Coumarin derivate, uncharged
  • Low molar mass: 343.42 g/mol
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications. 

ATTO 390 Datasheet

 ATTO 390 Fluorescent Dye Excitation and Emission Spectra Optical Properties:

λex = 390 nm

λem = 479 nm

εmax = 2.4×104

Φf = 0.90

τfl = 5.0 ns

Brightness = 21.6

Laser = 365 or 405 nm

 

APC (Allophycocyanin)
Overview:

  • High quantum yield
  • Large phycobiliprotein
  • 6 chromophores per molecule
  • Isolated from red algae
  • Molecular Weight: 105 kDa
  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

APC Datasheet

  APC Fluorophore Absorption and Emission Spectrum Optical Properties:

λex = 650 nm

λem = 660 nm

εmax = 7.0×105

Φf = 0.68

Brightness = 476

Laser = 594 or 633 nm

Filter set = Cy®5

 

Streptavidin

Properties:

  • Homo-tetrameric protein purified from Streptomyces avidinii which binds four biotin molecules with extremely high affinity
  • Molecular weight: 53 kDa
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA

Streptavidin Datasheet

BiotinBiotin Conjugate Structure

Properties:

  • Binds tetrameric avidin proteins including Streptavidin and neuravidin with very high affinity
  • Molar mass: 244.31 g/mol
  • Formula: C10H16N2O3S
  • Applications: Western blot, immunohistochemistry, and ELISA*

*The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

Biotin Datasheet

HRP (Horseradish peroxidase)

Properties:

  • Enzymatic activity is used to amplify weak signals and increase visibility of a target
  • Readily combines with hydrogen peroxide (H2O2) to form HRP-H2O2 complex which can oxidize various hydrogen donors
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. TMB, DAB, ABTS) into coloured products
    • Chemiluminescent substrates (e.g. luminol and isoluminol) into light emitting products via enhanced chemiluminescence (ECL)
    • Fluorogenic substrates (e.g. tyramine, homovanillic acid, and 4-hydroxyphenyl acetic acid) into fluorescent products
  • High turnover rate enables rapid generation of a strong signal
  • 44 kDa glycoprotein
  • Extinction coefficient: 100 (403 nm)
  • Applications: Western blot, immunohistochemistry, and ELISA*
    • *The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

HRP Datasheet

AP (Alkaline Phosphatase)

Properties:

  • Broad enzymatic activity for phosphate esters of alcohols, amines, pyrophosphate, and phenols
  • Commonly used to dephosphorylate the 5’-termini of DNA and RNA to prevent self-ligation
  • Catalyzes the conversion of:
    • Chromogenic substrates (e.g. pNPP, naphthol AS-TR phosphate, BCIP) into coloured products
    • Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate) into fluorescent products
  • Molecular weight: 140 kDa
  • Applications: Western blot, immunohistochemistry, and ELISA

AP Datasheet

  R-PE (R-Phycoerythrin)
Overview:

  • Broad excitation spectrum
  • High quantum yield
  • Photostable
  • Member of the phycobiliprotein family
  • Isolated from red algae
  • Excellent solubility in water
  • Molecular Weight: 250 kDa

 

  • Applications: The listed applications provide a general overview of potential uses for conjugated antibodies. However, they do not guarantee that every antibody-conjugate combination has been tested or validated for these specific applications.

R-PE Datasheet

  R-PE Fluorophore Excitation and Emission Spectra Optical Properties:

λex = 565 nm

λem = 575 nm

εmax = 2.0×106

Φf = 0.84

Brightness = 1.68 x 103

Laser = 488 to 561 nm

Filter set = TRITC

 
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Buffer PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
Storage Temperature -20ºC
Shipping Temperature Blue Ice or 4ºC
Purification Protein G Purified
Clonality Monoclonal
Clone Number 11D4
Isotype IgG1
Specificity Detects the N-terminal region of alpha synuclein
Cite This Product Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal (StressMarq Biosciences, Victoria BC, Cat# SMC-621)
Certificate of Analysis A 1:1000 dilution was sufficient for detection of Alpha Synuclein in rodent brain lysates in WB and 1:100 dilution on human brain tissue (Parkinson’s) in IHC.

Biological Description

Alternative Names Alpha Synuclein, α-Synuclein, SNCA, alphaSYN, NACP, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, Synuclein alpha, Synuclein alpha 140, Synuclein, alpha (non A4 component of amyloid precursor), isoform NACP140, PARK1, PARK 1, PARK4, PARK 4, Parkinson disease familial 1, Parkinson disease (autosomal dominant, Lewy body) 4, SYN, SYUA_HUMAN
Research Areas Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease, Synuclein, Tangles & Tau, Multiple System Atrophy
Cellular Localization Cytoplasm; membrane-bound to vesicles in neurons
Accession Number P37840
Gene ID 6622
Swiss Prot P37840
Scientific Background Alpha-synuclein is a presynaptic neuronal protein implicated in the pathogenesis of several neurodegenerative diseases, including Parkinson’s disease and dementia with Lewy bodies. Among its structural domains, the N-terminal region plays a pivotal role in membrane binding, conformational dynamics, and aggregation behavior. Post-translational modifications, particularly C-terminal truncations, significantly influence alpha-synuclein’s aggregation propensity and neurotoxicity (1).

C-terminally truncated alpha-synuclein species are highly enriched in pathological inclusions such as Lewy bodies and Lewy neurites. These truncated forms exhibit accelerated fibrillization and enhanced prion-like propagation in cellular and animal models of Parkinson’s disease, underscoring their pathogenic relevance (1). Importantly, such truncations can alter the structural conformation of alpha-synuclein, masking epitopes commonly targeted by antibodies, including those recognizing phosphorylated serine 129 (pSer129) .

This epitope masking has critical implications for research and diagnostics. Antibodies specific to pSer129 may fail to detect C-terminally truncated alpha-synuclein, potentially underestimating the burden of pathogenic species in tissue samples. Therefore, careful selection of antibodies that target the N-terminal region is essential for accurate detection and quantification of disease-relevant alpha-synuclein conformers (3).

Understanding the structural and functional roles of the alpha-synuclein N-terminus is vital for elucidating disease mechanisms and developing targeted therapeutic strategies in synucleinopathies.
References 1.,Mahul-Mellier, A.-L., Altay, M. F., Maharjan, N., et al. (2024). Dissecting the differential role of C-terminal truncations in the regulation of aSyn pathology formation and the biogenesis of Lewy bodies. bioRxiv. DOI: 10.1101/2024.11.29.625993
2.,Bell, R., Vendruscolo, M. (2021). Modulation of the interactions between α-synuclein and lipid membranes by post-translational modifications. Frontiers in Neurology, 12, 661117. DOI: 10.3389/fneur.2021.661117
3.,Iyer, A., Roeters, S. J., Kogan, V., Woutersen, S., Claessens, M. M. A. E., & Subramaniam, V. (2017). C-Terminal Truncated α-Synuclein Fibrils Contain Strongly Twisted β-Sheets. Journal of the American Chemical Society, 139(43), 15392-15400. DOI: 10.1021/jacs.7b07403

Product Images

<p>Western blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomers (SPR-321 & SPR-316). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) or Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 610-103-121) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 0.5 second.</p>

Western blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomers (SPR-321 & SPR-316). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) or Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 610-103-121) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 0.5 second.

<p>Immunohistochemistry analysis with Stressmarq’s Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). Tissue: Striatum sections of Parkinson’s Diseased Brain. Species: Human. Fixation: Paraffin embedded, 5um thin. Blocking: 3% milk for 1 hour at RT. Antigen Retrieval: Citrate buffer, pH 6.0, boiled for 20 min. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.</p>

Immunohistochemistry analysis with Stressmarq’s Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). Tissue: Striatum sections of Parkinson’s Diseased Brain. Species: Human. Fixation: Paraffin embedded, 5um thin. Blocking: 3% milk for 1 hour at RT. Antigen Retrieval: Citrate buffer, pH 6.0, boiled for 20 min. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-Terminal Monoclonal Antibody, Clone 11D4 (SMC-621). at 1:100 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (H&L) at 1:100 for 45 min at RT. Counterstain: Hematoxylin (purple) nuclear stain at 1:10 for 5 min at RT. Color Development: DAB at 1:50 for 15 min at RT.

<p>Dot blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 and SMC-532 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomer (SPR-321). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621), Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) or Mouse Anti-Mouse Alpha Synuclein Monoclonal (SMC-532) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 710-1331) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 1 second or 5 seconds.</p>

Dot blot analysis comparing Stressmarq’s SMC-621 with Stressmarq’s SPC-800 and SMC-532 showing differences in detection of Recombinant Human Alpha Synuclein aa 1-114 Monomer, Recombinant Human Alpha Synuclein-98 Monomer, and Recombinant Human Alpha Synuclein Full-length Monomer (SPR-321). Block: 5% skim milk for 1/2 hour at RT. Primary Antibodies: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621), Rabbit Anti-Human Alpha Synuclein Polyclonal (SPC-800) or Mouse Anti-Mouse Alpha Synuclein Monoclonal (SMC-532) at 1:1000 for 1/2 hour at RT. Secondary Antibodies: Goat anti-mouse IgG (Rockland Cat# 710-1331) or Goat anti-rabbit IgG (Rockland Cat# 711-1322) at 1:4000 for 1 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 1 min at RT in the dark. Exposed 1 second or 5 seconds.

<p>Western blot analysis with Stressmarq’s SMC-621 showing detection of Alpha Synuclein protein in rat brain and mouse brain lysates. Block: 5% skim milk for 1/2 hour at RT. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (Rockland Cat# 610-103-121) at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 3 seconds (A), cropped and followed by a 1 minute exposure (B) to develop the monomer band in the rat brain lysate.</p>

Western blot analysis with Stressmarq’s SMC-621 showing detection of Alpha Synuclein protein in rat brain and mouse brain lysates. Block: 5% skim milk for 1/2 hour at RT. Primary Antibody: Mouse Anti-Human Alpha Synuclein N-terminal Monoclonal [11D4] (SMC-621) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat anti-mouse IgG-HRP (Rockland Cat# 610-103-121) at 1:4000 for 1/2 hour at RT. Color Development: Chemiluminescent for HRP (Moss) for 3 min at RT in the dark. Exposed 3 seconds (A), cropped and followed by a 1 minute exposure (B) to develop the monomer band in the rat brain lysate.

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