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Properties
| Storage Buffer | PBS pH7.2, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated |
| Storage Temperature | -20ºC, Conjugated antibodies should be stored according to the product label |
| Shipping Temperature | Blue Ice or 4ºC |
| Purification | Protein G Purified |
| Clonality | Monoclonal |
| Clone Number | D7A |
| Isotype | IgG1 |
| Specificity | Recognizes 90kDa. Can isolate complexes of HSP90, Src kinase and cec37. |
| Cite This Product | HSP90 Antibody (StressMarq Biosciences | Victoria, BC CANADA, Catalog# SMC-137, RRID: AB_2121062) |
| Certificate of Analysis | 2 µg/ml was sufficient for detection of HSP90α in 20 µg of heat shocked HeLa cell lysate as well as in 100 ng of human HSP90α protein by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary. |
Biological Description
| Alternative Names | Heat shock protein 90 alpha, Heat shock protein 90‑alpha, Heat shock 90 kDa protein alpha, Hsp90α, Hsp90-alpha, HSP90AA1, HSP90A, HSPC1, HSP90N, HSPCAL4, HSPCA, Hsp86, Hsp90A1 |
| Research Areas | Cancer, Cell Signaling, Chaperone Proteins, Heat Shock, Protein Trafficking, Tumor Biomarkers |
| Cellular Localization | Cytoplasm, Melanosome |
| Accession Number | NP_001103255.1 |
| Gene ID | 423463 |
| Swiss Prot | P11501 |
| Scientific Background |
HSP90 is a highly conserved and abundantly expressed molecular chaperone that plays a central role in maintaining protein homeostasis, particularly in the nervous system. Present in all eukaryotic cells, HSP90 exists in two major cytosolic isoforms—HSP90α and HSP90β—which share 85% sequence identity but differ in oligomeric state and regulatory function. Despite its classification as a heat shock protein, HSP90 is constitutively expressed at high levels, comprising up to 2% of total cytosolic protein in unstressed cells. It is essential for the folding, maturation, and stabilization of a wide range of client proteins, many of which are involved in neuronal signaling, synaptic plasticity, and stress response. These include kinases (e.g., c-Raf), transcription factors (e.g., p53), and steroid hormone receptors. In neurodegenerative diseases such as Alzheimer’s, Parkinson’s, and Huntington’s, HSP90 is implicated in both protective and pathological processes. It stabilizes misfolded proteins and prevents aggregation, but can also shield aberrant proteins from degradation, contributing to disease progression. HSP90’s interaction with co-chaperones like Cdc37 and p23 forms complexes that regulate the fate of client proteins, making it a key node in proteostasis networks. Pharmacological inhibition of HSP90—using compounds like geldanamycin—has shown promise in modulating protein quality control pathways and reducing toxic protein accumulation in neurodegenerative models. |
| References |
1. Schuh S. et al. (1985) J Biol Chem. 260 (26): 14292-14296. 2. Lipsich L.A., Cutt J.R. and Brugge J.S. (1982) Mol. Cell Biol. 2(7): 875-880. 3. Brugge J.S., Yonemoto W., and Darrow D. (1983) Mol. Cell. Biol. 3(1): 9-19. 4. Arlander SJH, et al. (2003) J Biol Chem 278: 52572-52577. 5. Pearl H, et al. (2001) Adv Protein Chem 59: 157-186. 6. Neckers L, et al. (2002) Trends Mol Med 8:S55-S61. 7. Pratt W, Toft D. (2003) Exp Biol Med 228:111-133. 8. Pratt W, Toft D. (1997) Endocr Rev 18: 306–360. 9. Pratt WB. (1998) Proc Soc Exptl Biol Med 217: 420–434. 10. Whitesell L, et al. (1994) Proc Natl Acad Sci USA 91: 8324– 8328. |
Product Images
Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha (SMC-137). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-137) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.
Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7alpha (SMC-137). Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-137) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.
Western Blot analysis of Rat cell lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone D7Alpha (SMC-137). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody (SMC-137) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
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Based on validation through cited publications.