MOLECULAR SIGNATURE®
VPS35 Kit

Colorimetric measurement of VPS35

Catalog No. SKT-141

0 out of 5 based on 0 customer ratings
Species Reactivity Human, Mouse, Rat
Sample Types Cell Lysate, Tissue
SKU: SKT-141 Categories: ,

Clear
SKT-141_VPS35_Kit_Typical_Standard_Curve
Graph of the Linearity Recovery for the VPS35 Kit - SKT-141Graph of the VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit MOLECULAR SIGNATURE® - SKT-141
Product Name VPS35 Kit
Description

Colorimetric measurement of VPS35

Species Reactivity Human, Mouse, Rat
Platform Microplate
Sample Types Cell Lysate, Tissue
Detection Method Colorimetric
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Sensitivity 50 pg/mL
Assay Range 78.125 - 5000 pg/mL
Incubation Time 3 hours
Number of Samples 40 samples in duplicate
Other Resources Kit Booklet Lot No. SV999550 , Kit Booklet Lot No. SV888770 , MSDS
Field of Use Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Kit Overview
Component No.ItemQuantity / Size
SKC-141A Anti-VPS35 Immunoassay Plate 1 Plate
SKC-141B 5X VPS35 Extraction Reagent 1 bottle/10mL
SKC-141C Recombinant VPS35 Standard 2 vials
SKC-141D Standard and Sample Diluent (Red) 1 bottle/50mL
SKC-141E 10X Wash Buffer Concentrate 1 bottle/100mL
SKC-141F Anti-VPS35 Biotinylated Antibody Concentrate 1 vial/150uL
SKC-141G Anti-VPS35 Biotinylated Antibody Diluent (Green) 1 bottle/13mL
SKC-141H 100X Streptavidin:HRP Concentrate 1 vial/150uL
SKC-141I Streptavidin: HRP Diluent (Purple) 1 bottle/13mL
SKC-141J TMB Substrate 1 bottle/13mL
SKC-141K Stop Solution 1 bottle/13mL

Biological Description

Research Areas Alzheimer's Disease, Cell Signaling, Golgi Proteins, Membrane Trafficking Proteins, Neurodegeneration, Neuroscience, Parkinson's Disease, Protein Trafficking
Scientific Background Vacuolar Protein Sorter-35 (VPS35) is a component of the retromer complex, which is essential for endosome-to-Golgi retrieval of membrane proteins. VPS35 mutations such as D620N have been linked to Parkinson’s Disease (PD) (1,2) and affect retromer function, protein homeostasis, and mitochondria (3).
References 1. Vilarino-Guell, C. et al. (2011) Am J Hum Genet 89:162–167
2. Zimprich, A. et al. (2011) Am J Hum Genet 89:168–175
3. Rahman, A.A., Morrison, B.E. (2019) Neurosci 401:1-10.

Product Images

<p>Typical Standard Curve for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.</p>

Typical Standard Curve for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.

<p>VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. </p>

VPS35 Determination of WT-A549 vs KN-549 Lysates Graph for the VPS35 Kit (Enzyme-Linked Immunosorbent Assay) MOLECULAR SIGNATURE® – SKT-141. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay.

<p>Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions. </p>

Linearity was determined by using low (rat kidney) and high (mouse kidney) natural samples, diluted 1/100 and mixed in various proportions.

Reviews

Reviews

There are no reviews yet.

Be the first to review “VPS35 Kit”

Your email address will not be published. Required fields are marked *