|Product Name||Alpha Synuclein Protein|
Active Human Recombinant Alpha Synuclein Protein Monomer
|Applications||WB, SDS-PAGE, In vivo assay, In vitro assay|
|Concentration||Lot/batch specific. See included datasheet.|
HRP (Horseradish peroxidase)
AP (Alkaline Phosphatase)
|Expression System||E. coli|
|Amino Acid Sequence||MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA|
|Protein Length||Full Length|
|Full Biological Activity||100 µM alpha synuclein protein monomer (SPR-321) seeded with 10 nM alpha synuclein protein aggregate (SPR-322) in 25 µM Thioflavin T (PBS pH 7.4, 100 µl reaction volume) generated a fluorescence intensity of 13,000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 24 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.|
|Shipping Temperature||Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.|
|Cite This Product||Human Recombinant Alpha Synuclein Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-321)|
|Certificate of Analysis||Certified >95% pure using SDS-PAGE analysis.|
|Alternative Names||Active Alpha synuclein monomer, Active Alpha-synuclein monomer, Active Alpha synuclein protein monomer, Active Alpha synuclein monomer, Active Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Active Alpha synuclein monomers, SYN protein, Parkison disease familial 1 Protein|
|Research Areas||Alzheimer's Disease, Neurodegeneration, Neuroscience, Parkinson's Disease|
|Cellular Localization||Cytoplasm, Membrane, Nucleus|
|Scientific Background||Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson’s disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).|
1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013).
2. Zhang L., et al. (2008) Brain Res. 1244: 40-52.
3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117.
4. Kokhan V.S., Afanasyeva M.A., Van’kin G. (2012) Behav. Brain. Res. 231(1): 226-230.
5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840.
6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.
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