|Product Name||GRP78 (Bip) Protein|
Active Human Recombinant GRP78 (Bip) Protein
|Applications||WB, SDS-PAGE, ATPase Activity Assay, Functional Assay, ELISA|
|Concentration||Lot/batch specific. See included datasheet.|
HRP (Horseradish peroxidase)
AP (Alkaline Phosphatase)
|Expression System||E. coli|
|Amino Acid Sequence||MKLSLVAAMLLLLSAARAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRII|
|Full Biological Activity||ATPase active|
|Storage Buffer||20mM Tris, 50mM KCl, 5mM MgCl2, 1mM DTT, pH7.5|
|Shipping Temperature||Blue Ice or 4ºC|
|Cite This Product||Human Recombinant GRP78 (Bip) Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-107)|
|Certificate of Analysis||This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 2.3µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH 8) in the presence of 20ul of 1mM ATP using a Malachite Green assay.|
|Alternative Names||BIP Protein, Grp78 Protein, HSPA5 Protein, MIF2 Protein, immunoglobulin heavy chain binding Protein|
|Research Areas||Cancer, Heat Shock, Cell Signaling, Chaperones, Organelle Markers, Trafficking|
|Cellular Localization||Endoplasmic reticulum lumen, Melanosome|
|Scientific Background||GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' non-translated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia (Barnes), to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).|
1. Cho S., et al (2007) Mol Cell Biol. 27(1): 368-83.
2. Yang Y., et al. (1998) J Biol Chem. 273: 25552-25555.
3. Luo S., et al (2006) 26(15): 5688-97.
4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314.
5. Koomagi R., et al. (1999) Anticancer Res. 19: 4333-4336.
6. Laquerre S., et al. (1998) J. Virology. 72: 4940-4949.
7. Dong D., et al. (2005) Cancer Res. 65(13): 5785-91.
Fitter, S., Gronthos, S., Ooi, S.S. and Zannettino, A.C. (2016) Stem Cells. 35(4):940-951.
PubMed ID: 28026090 Applications: Western Blot
Ciplys, E., Au Ynait, A., Slibinskas, R. (2014) Microb Cell Fact. 13(1):22.
PubMed ID: 24512104 Applications: ATPase activity assay
Chen, M. et al. (2014) Cell Death Differ. 21(5):797-810.
PubMed ID: 24464222 Applications: ELISA
Zwang, N.A. et al. (2009) J Proteome Res. 8 (3): 1540-1554.
PubMed ID: 19209902 Applications: Western Blot Control