|Product Name||HSP70 (Fish) Antibody|
Rabbit Anti-Salmon HSP70 (Fish) Polyclonal
|Species Reactivity||Brook Trout (Salvelinus fontinalis), Fish, Rainbow Trout (Oncorhynchus mykiss), Salmon, Three-spined stickleback (Gasterosteus aculeatus)|
|Antibody Dilution||WB (1:5000); optimal dilutions for assays should be determined by the user.|
|Immunogen||Synthetic peptide conjugated to KLH. Peptide sequence specific to C-terminal part of salmonid HSP70. The target is not present in any other HSP70 proteins.|
AP (Alkaline Phosphatase)
HRP (Horseradish peroxidase)
|Storage Buffer||Lyophilized rabbit Antiserum. For reconstitution add 100 µl of sterile water.|
|Shipping Temperature||Blue Ice or 4ºC|
|Specificity||Detects ~70kDa. It does not cross react with HSC70. It does not detect HSP70 in other species.|
|Cite This Product||Rabbit Anti-Salmon HSP70 Polyclonal (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPC-313)|
|Certificate of Analysis||0.2 µl/ml of SPC-313 was sufficient for detection of HSP70 in 20 µg of heat shocked (25°C) rainbow trout cytoplasmic fraction lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.|
|Alternative Names||HSP70 1 Antibody, HSP70 2 Antibody, HSP70.1 Antibody, HSP72 Antibody, HSPA1 Antibody, HSPA1A Antibody, HSPA1B Antibody|
|Research Areas||Cancer, Heat Shock|
|Scientific Background||HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). When cells are subjected to metabolic stress (e.g., heat shock) a member of the HSP 70 family, HSP 70 (HSP72), is expressed; HSP 70 is highly related to HSC70 (>90% sequence identity). Constitutively expressed HSC70 rapidly forms a stable complex with the highly inducible HSP70 in cells following heat shock. The interaction of HSC70 with HSP 70 is regulated by ATP. These two heat shock proteins move together in the cell experiencing stress. Furthermore, research on HSC70 has implicates it with a role in facilitating the recovery of centrosomal structure and function after heat shock (5). Looking for more information on HSP70? Visit our new HSP70 Scientific Resource Guide at http://www.HSP70.com.|
|References||1. Boorstein W.R., Ziegelhoffer T., and Craig E.A. (1993) J. Mol. Evol. 38(1): 1-17.
2. Rothman J. (1989) Cell. 59: 591-601.
3. DeLuca-Flaherty et al. (1990) Cell. 62: 875-887.
4. Bork P., Sander C., and Valencia A. (1992) Proc. Natl Acad. Sci. USA. 89: 7290-7294.
5. Brown C.L. et al. (1996) J. Biol. Chem. 271(2): 833-840.
Teigen, L.E., Orczewska, J.I., McLaughlin, J. (2015) Comp Biochem Physiol A Mol Integr Physiol. 188:139-47.
PubMed ID: 26123780 Reactivity: Gasterosteus aculeatus (Three-spined stickleback) Applications: Western Blot
Ings, J.S., Oakes, K.D., Vijayan, M.M., and Servos, M.R. (2012) Comp Biochem Physiol C Toxicol Pharmacol. 156 (2): 67-74.
PubMed ID: 22579662 Reactivity: Trout Applications: Western Blot
Ings, J.S., Servos, M.R. and Vijayan, M.M. (2011) Environ Sci Technol. 45 (6): 2368-2376.
PubMed ID: 21322548 Reactivity: Trout Applications: Western Blot
Faught, E., Henrickson, L. and Vijayan, M.M. (2016) J Endocrinol. 232(2):237-246.
PubMed ID: 27872197 Reactivity: Rainbow Trout