StressXpress®
Rat GRP78 ELISA Kit

Colorimetric detection of GRP78 in rat lysates

Catalog No. SKT-137

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Species Reactivity , Rt
Sample Types Tissue

USD $560.00

SKU: SKT-137 Categories: , .

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Overview

Product Name Rat GRP78 ELISA Kit
Description

Colorimetric detection of GRP78 in rat lysates

Species Reactivity Rat
Platform Microplate
Sample Types Tissue
Detection Method Colorimetric Assay
Assay Type Sandwich ELISA (Enzyme-linked Immunosorbent Assay)
Utility ELISA kit used to quantitate GRP78 concentration in rat samples.
Sensitivity 0.15 ng/ml
Assay Range 0 - 50 ng/ml
Precision Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate. The intra-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <10%.
Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays. The inter-assay coefficient of variation of the Nitrotyrosine ELISA has been determined to be <15%.
Incubation Time 30 minutes
Number of Samples 40 samples in duplicate
Other Resources , Kit Booklet , MSDS

Properties

Storage Temperature 4ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Assay Overview 1. Prepare Standard and samples in Standard and Sample Diluent.
2. Add 100 µL of Standard or sample to appropriate wells.
3. Cover plate with Plate Sealer and incubate at 37°C for 2 hours.
4. Wash plate four times with 1X Wash Buffer.
5. Add 100 µL of Biotinylated Antibody Working Solution to each well.
6. Cover plate with Plate Sealer and incubate at at 37°C for 2 hours.
7. Wash plate four times with 1X Wash Buffer.
8. Add 100 µL of Streptavidin Poly HRP Working Solution to each well.
9. Cover plate with Plate Sealer and incubate at 37°C for 30 minutes.
10. Wash plate four times with 1X Wash Buffer.
11. Add 100 µL of TMB Substrate to each well.
12. Develop the plate in the dark at room temperature for 30 minutes.
13. Stop reaction by adding 100 µL of Stop Solution to each well.
14. Measure absorbance on a plate reader at 450 nm.
Kit Components
Component No. Item Quantity / Size
SKC-137A Anti-Rat GRP78 Immunoassay Plate 1 Plate
SKC-137B Recombinant Rat GRP78 Standard 2 vials/ 56.7ng
SKC-0001 Standard and Sample Diluent 1 vial/50mL
SKC-0002 Anti-Rat Grp78 Biotinylated Antibody Concentrate 1 vial/150µL
SKC-0003 10X Wash Buffer Concentrate 1vial/100mL
SKC-137F Anti-Rat GRP78 Biotinylated Antibody Diluent 1 vial/13mL
SKC-137G Streptavidin Poly HRP Concentrate 1 vial/150µL
SKC-137H Streptavidin Poly HRP Diluent 1 vial/13mL
SKC-0004 TMB Substrate 1 vial/13mL
SKC-0005 Stop Solution 1 vial/13mL
SKC-0009 Plate Cover 2
Cite This Product Rat GRP78 ELISA Kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-137)

Biological Description

Alternative Names Grp78 ELISA Kit, Binding immunoglobulin protein ELISA Kit, BiP ELISA Kit, 78 kDa glucose-regulated protein ELISA Kit, heat shock 70 kDa protein 5 ELISA Kit
Research Areas Cancer, Heat Shock, Cell Signaling, Chaperones, Organelle Markers, Trafficking
Scientific Background GRP78 is a ubiquitously expressed, 78-kDa glucose-regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5’ nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1).
GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3).
In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti­tumor drugs (6, 7).
References 1. Cho S., et al. (2007). Mol Cell Biol 27(1): 368-83.
2. Yang Y., et al. (1998) J Biol Chem 273: 25552-25555.
3. Luo S., et al (2006) 26 (15): 5688-97.
4. Yu Z., et al. (1999) Exp Neurol. 15: 302-314.
5. Koomagi R., et al. (1999) Anticancer Res. 19:4333- 4336.
6. Laquerre S., et al. (1998) J. Virology 72: 4940-4949.
7. Dong D., et al. (2005) Cancer Res 65(13): 5785-91.

Product Images

Typical Standard Curve for the Rat GRP78 ELISA Kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-137. Assay Type: Sandwich ELISA. Detection Method: Colorimetric Assay. Assay Range: 0 – 50 ng/ml.

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