StressXpress®
DNA Damage (8-OHdG) ELISA kit

Colorimetric detection of 8-hydroxy-2-deoxy Guanosine

Catalog No. SKT-120

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Species Reactivity , ALL
Sample Types Cell lysates, Plasma, Sample matrices, Urine

USD $391.00

SKU: SKT-120 Categories: , .

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SKT-120_DNA_Damage_8-OHdG_ELISA_kit_Standard_Curve_Fig6.png
Chemical Equation of the Oxidation of Guanosine for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Diagram of the 8-OHdG Competitive ELISA for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Graph of the Urine Spike Assay for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Diagram of the Preparation of the 8-OHdG Standards for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Diagram of the Triplicate Sample Plate Format for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120Preview of the Calculations Worksheet for the DNA Damage (8-OHdG) ELISA kit StressXpress - SKT-120

Overview

Product Name DNA Damage (8-OHdG) ELISA kit
Description

Colorimetric detection of 8-hydroxy-2-deoxy Guanosine

Species Reactivity Species Independent
Platform Microplate
Sample Types Cell lysates, Plasma, Sample matrices, Urine
Detection Method Colorimetric Assay
Assay Type Competitive ELISA (Enzyme-linked Immunosorbent Assay)
Utility ELISA Kit for 8-OHdG detection in samples.
Sensitivity 0.59 ng/mL
Assay Range 0.94 - 60 ng/mL
Precision Intra-Assay Precision: Three samples of known concentration were assayed thirty times on one plate; the intra-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%. Inter-Assay Precision: Three samples of known concentration were assayed thirty times in three individual assays; the inter-assay coefficient of variation of the DNA Damage ELISA has been determined to be <5%.
Incubation Time 1 hour
Number of Samples 39 samples in duplicate
Other Resources , Kit Booklet , MSDS , Calculations Worksheet

Properties

Storage Temperature 4ºC and -20ºC
Shipping Temperature Blue Ice
Product Type ELISA Kits
Assay Overview 1. Prepare standard and samples in the Sample and Standard Diluent.
2. Add 50 µL of prepared standards and samples in triplicate to appropriate wells.
3. Add 50 µL of the diluted antibody preparation to the appropriate wells.
4. Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour.
5. Wash plate 4 times with 1X Wash Buffer.
6. Add 100 µL of TMB Substrate to each well.
7. Cover plate and develop the plate in the dark at room temperature for 30 minutes.
8. Add 100 µL of Stop Solution to each well.
9. Measure absorbance on a plate reader at 450 nm.
10. Plot the standard curve and calculate sample concentrations.
Kit Components
Component No. Item Quantity / Size
SKC-120A 8-hydroxy-2-deoxy Guanosine : BSA Coated Plate 1 Plate
SKC-120C 8-hydroxy-2-deoxy Guanosine Standard 1 vial/ 100uL
SKC-120F 8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody 1 vial/75uL
SKC-0001 Sample and Standard Diluent 1 vial/50mL
SKC-0002 8-hydroxy-2-deoxy Guanosine Antibody Diluent 1 vial/13mL
SKC-0003 Wash Buffer Concentrate 1 vial/50mL
SKC-0004 TMB Substrate 1 vial/13mL
SKC-0005 Stop Solution 1 vial/13mL
SKC-0009 Plate Cover  2 covers
Cite This Product DNA Damage (8-OHdG) ELISA kit (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SKT-120)

Biological Description

Alternative Names 8-OH-dG ELISA Kit, 8OHG ELISA Kit, 80G ELISA Kit, 8 hydroxyguanine ELISA Kit, 8-OHdG ELISA Kit, DNA Damage ELISA Kit
Research Areas Cancer, Cell Signaling, Oxidation, Oxidative Stress, Post-translational Modifications
Scientific Background 8-hydroxy-2-deoxy Guanosine (8-OH-dG) is produced by the oxidative damage of DNA by reactive oxygen and nitrogen species and serves as an established marker of oxidative stress (1-4). Hydroxylation of guanosine occurs in response to both normal metabolic processes and a variety of environmental factors (i.e., anything that increases reactive oxygen and nitrogen species). Increased levels of 8-OH-dG are associated with the aging process as well as with a number of pathological conditions including cancer, diabetes, and hypertension(5-9). In complex samples such as plasma, cell lysates, and tissues, 8-OH-dG can exist as either the free nucleoside or incorporated in DNA. Once the blood enters the kidney, free 8-OH-dG is readily filtered into the urine, while larger DNA fragments remain in the bloodstream. Because of the complexity of plasma samples, urine is a more suitable matrix for the measurement of free 8-OH-dG than plasma. Urinary levels of 8-OH-dG range between 2.7-13 ng/mg creatine, while plasma levels of free 8-OH-dG have been reported to be between 4-21 pg/ml as determined by LC-MS (10-11).
References 1. Maxey K.M., Maddipati K.R., Birkmeier J. (1992) J Clin Immunoassay 15: 16-120.
2. Pradelles P., Grassi J., Maclouf J. (1990) Methods Enzymol. 187: 24-34.
3. Maclouf J., Grassi J., Pradelles P. (1987) Dev Immunoassay Tech Meas eicosanoids.
4. Lin H., et al. (2004) Biochem J. 380: 541-548.
5. Bogdanov M.B., et al. (1999) Free Radic Biol Med. 27(5/6): 647-666.
6. Lee J., et al. (2005) Hypertension 45: 986-990.
7. Leinonen, J., et al. (1997) FEBSLett. 417: 150-152.
8. Endo K., et al. (2006) J. Atheroscler. Thromb. 13:68-75.
9. Kuo H., et al. (2007) Mutat Res. 631:62-68.
10. Shen J., et al. (2007) Cancer 109: 574-580.
11. Beckman K.B., Ames B.N. (1997) J Biol Chem 272: 19633-19636.
12. Epe B., et al. (1996) Nucleic Acids Res 24: 4105-4110.
13. Spencer J.P.E., et al. (1995) FEBS Lett 374: 233-236.
14. Floyd R.A. (1990) FASEB J 4: 2587-2597.

Product Images

Preview of the Calculations Worksheet

Diagram of the Triplicate Sample Plate Format

Diagram of the Preparation of the 8-OHdG Standards

Urine Spike Assay

Diagram of the 8-OHdG Competitive ELISA

Chemical Equation of the Oxidation of Guanosine

Typical Standard Curve for the DNA Damage (8-OHdG) ELISA kit (Enzyme-Linked Immunosorbent Assay) StressXpress® – SKT-120. Assay Type: Competitive ELISA. Detection Method: Colorimetric Assay. Assay Range: 0.94 – 60 ng/ml.

Product Citations (39)

Other Citations

Coagulin-l ameliorates TLR4 induced oxidative damage and immune response by regulating mitochondria and NOX-derived ROS.

Reddy, SS (2016) Toxicol Appl Pharmacol. [Epub ahead of print].

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Preventive Effect of Resveratrol against Brain Mitochondria DNA Damage, Lipid Peroxidation, Inflammation and Seizures Induced by Kainic Acid in Mice.

Hussein, S. A., Abdel-mageid, A.D., Abd-Elhamed, O. M., Amin, A. and Al harthy, H.S. (2016) Int J Phar Sci. 6(4): 1634-1646

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Does chronic raise of metal ion levels induce oxidative DNA damage and hypoxia-like response in patients with metal-on-metal hip resurfacing?

Savarino, L. et al. (2015) J Biomed Mater Res B Appl Biomater. [Epub ahead of print].

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Higher urinary Levels of 8-hydroxy-2'-deoxyguanosine are associated with a worse RANKL/OPG ratio in postmenopausal women with osteopenia.

Cervellati, C. et al. (2015) Oxid Med Cell Long. [Epub ahead of print].

PubMed ID: Reactivity: Human

Synthesis, characterization and biological activity of some unsymmetrical Schiff base transition metal complexes.

Esmadi, F.T. et al. (2015) Drug Chem Toxicol. :1-7.

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Late-intervention study with ebselen in an experimental model of type 1 diabetic nephropathy.

Tan, S.M., Sharma, A., Stefanovic, N. and de Haan, J.B. (2015) Free Radic Res. 49(3):219-27.

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Immediate and delayed effects of growth conditions on ageing parameters in nestling zebra finches.

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Stier, A., et al. (2014) Oecologia. 175(3):791-800.

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Evaluation of vitamin B12 effects on DNA damage induced by paclitaxel.

Alzoubi, K., Khabour O., Khader M., Mhaidat N. and Al-Azzam, S. (2014) Drug Chem Toxicol. 37(3):276-80.

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Disruption of pro-oxidant and antioxidant systems with elevated expression of the ubiquitin proteosome system in the cachectic heart muscle of nude mice.

Hinch, E. C. A., Sullivan-Gunn, M. J., Vaughan V. C., McGlynn M. A., Lewandowski, Paul A. (2013) J Cachexia Sarcopenia Muscle. 4(4):287-93.

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Differential effects of docosahexanoic acid (DHA) on preterm and term placental pro-oxidant/anti-oxidant balance.

Stark, M. et al. (2013) Reproduction. 146(3):243-51.

PubMed ID: 23813449 Reactivity: Human

TiO2 Nanoparticle Exposure and Illumination during Zebrafish Development: Mortality at Parts per Billion Concentrations.

Bar-Ilan, O. et al. (2013) Environ Sci Technol. 47 (9): 4726-4733.

PubMed ID: 23510150 Reactivity: Zebrafish

Poor sleep in PCOS; is melatonin the culprit?

Shreeve, N. et al. (2013) Hum Reprod. 28 (5): 1348-1353.

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Grasshoppers' adaptation to elevated radioactivity in the Chernobyl exclusion zone.

Mortensen, L.H. (2013) Roskilde University Dissertation

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Consumption of a low glycaemic index diet in late life extends lifespan of Balb/c mice with differential effects on DNA damage.

Nankervis, S.A., Mitchell, J.M., Charchar, F.J., McGlynn, M.A. and Lewandowski, P.A. (2013) Longev Healthspan. 2(1):4.

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Light-induced cell detachment for cell sheet technology.

Hong, Y. et al. (2013) Biomaterials. 34 (1): 11-18.

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Puerarin prevents high glucose-induced apoptosis of Schwann cells by inhibiting oxidative stress.

Wu, Y., Xue, B., Li, X. and Liu, H. (2012) Neural Regen Res. 7 (33): 2583-2591.

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The protective effect of Alpha lipoic acid on Schwann cells exposed to constant or intermittent high glucose.

Sun, L. et al. (2012) Biochem Pharmacol. 84 (7): 961-973.

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Xue, B. et al. (2012) Neural Regen Res. 2012 (30): 2340-2346.

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Vaughan, V.C., Sullivan-Gunn, M., Hinch, E., Martin, P., Lewandowski, P.A. (2012) PLoS ONE. 7(9): e45900.

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Titanium dioxide nanoparticles produce phototoxicity in the developing zebrafish.

Bar-Ilan, O. et al. (2012) Nanotoxicology. 6 (6): 670-679.

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Evaluation of vitamin B12 effects on DNA damage induced by pioglitazone.

Alzoubi, K., Khabour, O., Hussain, N., Al-azzam, S. and Mhaidat, N. (2012) Mutat Res. 748 (1-2): 48-51.

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Protective Effects of Salvianolic Acid B on Schwann Cells Apoptosis Induced by High Glucose.

Sun, L. et al. (2012) Neurochemical Res. 37 (5): 996-1010.

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A qPCR-based assay to quantify oxidized guanine and other FPG-sensitive base lesions within telomeric DNA.

O'Callaghan, N., Baack, N., Sharif, R. and French, M. (2011) Biotechniques. 51 (6): 403-411.

PubMed ID: 22150331 Reactivity: Human

The effect of cocoa supplementation on hepatic steatosis, reactive oxygen species and LFABP in a rat model of NASH.

Janevski, M., Antonas, K.N., Sullivan-Gunn, M.J., McGlynn, M.A. and Lewandowski, P.A. (2011) Comp Hepatol. 10(1):10.

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Reduction of Oxidative Damage Reflects a Better Kidney Transplantation Outcome. 

La Manna, G. et al.  (2011) Am J Nephrol. 34, 496-504. 

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